Multiplexed Digital Assay Combinatorial Signal Detection
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current digital assays face limitations in multiplexing capabilities due to the high cost and impracticality of increasing the number of distinguishable dyes and detection channels, which restricts the ability to detect multiple targets simultaneously, especially in applications with limited sample volumes.
Innovation Solution
A color-based approach for multiplexed digital amplification assays that uses combinatorial signaling, where multiple targets are amplified in the same partitions, and composite signals are generated across different wavelength regimes to detect the presence of multiple targets using fewer detection channels, allowing for the calculation of individual target concentrations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If the number of distinguishable dyes and detection channels is increased to detect more targets, then the multiplexing capability is improved, but the cost and practicality deteriorate
Solution Approach 1:
The patent combines multiple detection channels by using spectral unmixing algorithms that analyze composite fluorescence signals containing contributions from multiple dyes. Instead of requiring separate detection channels for each dye, the system merges the detection into fewer channels and uses computational methods to resolve the individual dye signals based on their spectral characteristics.
Solution Approach 2:
The patent transitions from spatial separation of detection channels to spectral dimension analysis. By measuring fluorescence intensities across multiple wavelengths in fewer detection channels and applying spectral unmixing, the system extracts information about multiple dyes without requiring an equal number of physical detection channels.
2Adaptability or versatility
If the number of distinguishable dyes is increased to detect more targets, then the multiplexing capability is improved, but the cost and practicality deteriorate
Solution Approach 1:
The patent combines multiple detection channels by using spectral unmixing algorithms that analyze composite fluorescence signals containing contributions from multiple dyes. Instead of requiring separate detection channels for each dye, the system merges the detection into fewer channels and uses computational methods to resolve the individual dye signals based on their spectral characteristics.
Solution Approach 2:
The patent uses reference spectral data from individual dyes to create a computational model that can deconvolve composite signals. By having spectral fingerprints of individual dyes stored as references, the system can mathematically separate the contributions of multiple dyes in a composite signal, effectively creating virtual detection channels through computational copying of spectral information.
3Measurement precision
If more detection channels are used to detect more colors, then the accuracy of target detection is improved, but the cost and complexity increase
Solution Approach 1:
The patent transitions from spatial separation of detection channels to spectral dimension analysis. By measuring fluorescence intensities across multiple wavelengths in fewer detection channels and applying spectral unmixing, the system extracts information about multiple dyes without requiring an equal number of physical detection channels.
Solution Approach 2:
The patent employs iterative spectral unmixing algorithms that use feedback from measured composite signals and reference spectral data to progressively resolve the contributions of individual dyes. The system compares measured signals against expected spectral patterns and adjusts the decomposition to maximize agreement between observed and predicted signals, thereby maintaining detection accuracy.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables the detection of multiple targets in a single assay without the need for additional detection channels, increasing multiplexing capacity while reducing costs and sample volume requirements, thereby enhancing the analytical capabilities of digital assays.
Implementation Method 1
the probe can include a dye that provides a fluorescence signal indicating whether or not the target has been amplified
Data Source
Figure 1~2
Figure 3~5
Figure 6~8
AI summary
System, including methods, apparatus, and compositions, for performing a multiplexed digital assay on a greater number of targets through combinatorial use of signals.