Multiplexed Digital Assay Signal Merging
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Solution Overview
Problem
Current digital assays face limitations in multiplexing levels due to the need for more optical channels to detect multiple targets, which is costly and not feasible for applications with limited sample volumes.
Innovation Solution
The system enables multiplexed digital assays by collecting signals from two or more distinct targets in a common optical channel using probes with similar spectral characteristics, allowing for the simultaneous detection of multiple targets through distinct endpoint assays or time courses, and using Poisson statistics to estimate target concentrations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If more optical channels are used to detect multiple targets with different dyes, then the multiplexing level increases, but the instrument cost increases
Solution Approach 1:
The patent combines signals from multiple targets that would traditionally require separate optical channels into a single common optical channel. By using probes with similar spectral characteristics and collecting their signals together, the system achieves multiplexed detection without requiring proportional increases in optical channels, thereby reducing instrument cost while maintaining high multiplexing levels
Solution Approach 2:
The patent introduces temporal dimension by utilizing distinct endpoint assays or time courses to differentiate between targets detected in the same optical channel. This allows the system to resolve multiple targets along the time axis rather than requiring separate spatial (optical channel) dimensions, effectively increasing multiplexing capability without proportionally increasing instrument complexity
2Adaptability or versatility
If more optical channels are used to detect multiple targets, then the detection capability increases, but the sample volume requirement increases
Solution Approach 1:
The patent merges the detection of multiple targets into a single optical channel, which means all targets are detected within the same sample partitions. This eliminates the need to allocate separate sample volumes for each optical channel, thereby maintaining high detection capability while significantly reducing the total sample volume required
Solution Approach 2:
The single optical channel is designed to perform multiple detection functions simultaneously for different targets. By making the optical channel universal rather than dedicated to a single target, the system maximizes the utilization of the limited sample volume across all target detections
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for the detection of multiple targets in fewer optical channels, increasing multiplexing levels without the need for expensive instrumentation, thereby enhancing the analysis of genetic material and reducing sample requirements.
Implementation Method 1
the probe can include a dye that provides a fluorescence signal indicating whether or not the target has been amplified
Data Source
AI summary
System, including methods and apparatus, for performing a multiplexed digital assay.