Multiplexed Electrochemical Impedance Sensor for Biomarker Detection
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Solution Overview
Problem
Current diagnostic methods for monitoring multiple biomarkers require multiple sensors, assays, and often involve labeling, which can lead to user error and are not suitable for daily monitoring due to the need for multiple finger pricks, especially in conditions like diabetes and cancer, where sensitivity and specificity are limited by single biomarkers.
Innovation Solution
A multi-marker electrochemical impedance spectroscopy sensor with molecular recognition elements conjugated to tuning elements, such as nanoparticles, allowing for distinct frequency alteration and detection of multiple biomarkers on a single sensor, enabling label-free, multiplexed assays.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If multiple sensors and assays are used to detect multiple biomarkers, then detection capability is improved, but device complexity and ease of operation deteriorate
Solution Approach 1:
The patent combines multiple molecular recognition elements (different antibodies) onto a single sensor surface, allowing simultaneous detection of multiple biomarkers (PSA, hPSA, fPSA) without requiring separate sensors or assays. This merging approach maintains high detection capability while reducing device complexity and improving ease of operation.
Solution Approach 2:
The sensor is designed with universal functionality to detect multiple different biomarkers using a single platform. The sensor surface can accommodate various molecular recognition elements that recognize different targets, making the device multi-functional and eliminating the need for multiple specialized sensors.
2Measurement precision
If labeling is used in assays, then detection sensitivity is improved, but ease of operation and reliability deteriorate due to user error
Solution Approach 1:
The assay system is designed to be self-service, automatically performing detection without requiring manual labeling steps by the user. The molecular recognition elements are pre-immobilized on the sensor surface with optimized configurations that enable direct detection, eliminating user error-prone labeling operations while maintaining high detection sensitivity.
3Adaptability or versatility
If multiple finger pricks are required for monitoring, then detection capability is improved, but ease of operation and patient compliance deteriorate
Solution Approach 1:
The invention merges multiple detection capabilities into a single sensor platform that can analyze multiple biomarkers from one blood sample. This eliminates the need for multiple finger pricks required by separate assays, thereby improving patient compliance and ease of operation while maintaining comprehensive detection capability.
4Device complexity
If single biomarker assays are used, then device complexity is reduced, but measurement precision and adaptability deteriorate
Solution Approach 1:
The sensor employs universal molecular recognition elements that can simultaneously detect multiple biomarkers with high precision. The single sensor platform maintains low device complexity while achieving high measurement precision through the use of multiple specific antibodies immobilized on the same surface, enabling comprehensive diagnostic accuracy.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for the sensitive and specific detection of multiple biomarkers in a single assay, improving diagnostic accuracy and reducing the need for multiple sensors, thereby enhancing patient compliance and monitoring efficiency.
Implementation Method 1
A multi-marker electrochemical impedance spectroscopy sensor with molecular recognition elements conjugated to tuning elements, such as nanoparticles, allowing for distinct frequency alteration and detection of multiple biomarkers on a single sensor
Data Source
AI summary
The present invention relates to a diagnostic device and methods of using the same for diagnostic assays for monitoring the presence of biological samples wherein the device allows for the determination of at least two assay components on one sensor. More specifically, the invention relates to a multi-marker electrochemical impedance spectroscopy sensor comprising a plurality of molecular recognition elements wherein the sensor comprises multiple different molecular recognition element types that are tuned in a manner that alters the frequency of the molecular recognition element type such that it is at a detectably different frequency to the frequency of other molecular recognition element types on the same sensor.