Multi-Wavelength Absorbance Correction for Mixed Liquid Concentration
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Solution Overview
Problem
Conventional concentration measurement methods for mixed solutions, particularly cell suspensions, face inaccuracies due to varying impurity levels, leading to incorrect concentration calculations.
Innovation Solution
A concentration measurement device that measures absorbance in multiple wavelength regions and corrects concentrations based on these measurements, using a calibration curve created with a known mixed liquid, to account for different liquid components.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If a calibration curve is prepared in advance for concentration measurement, then measurement process is simplified, but measurement accuracy deteriorates when impurity levels vary
Solution Approach 1:
The patent transitions from single-wavelength absorbance measurement to multi-wavelength spectral measurement. By measuring absorbance across multiple wavelengths and analyzing spectral characteristics, the system can distinguish between target substances and impurities, thereby maintaining measurement simplicity while improving accuracy through dimensional expansion in the spectral domain
Solution Approach 2:
The patent changes the measurement parameter from single-wavelength absorbance to multi-wavelength spectral absorbance. By utilizing absorbance values at different wavelengths and their relationships, the system can correct for impurity interference and accurately determine target substance concentrations even when impurity levels vary
2Device complexity
If single wavelength absorbance measurement is used, then measurement device is simple, but measurement accuracy deteriorates due to impurity interference
Solution Approach 1:
The patent expands the measurement from a single wavelength point to multiple wavelength points, creating a spectral fingerprint for the sample. This dimensional expansion allows the system to identify and compensate for impurity interference patterns, improving accuracy without requiring complex sample preparation or separation techniques
Solution Approach 2:
The patent introduces spectral ratio or differential absorbance values at different wavelengths as intermediary parameters. These intermediaries serve as indicators that help distinguish target substance signals from impurity signals, enabling accurate concentration measurement while keeping the device relatively simple
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Improves measurement accuracy by accurately determining the concentration of mixed liquids, even when containing impurities, by correcting absorbance based on mixing ratios and using a calibration curve.
Implementation Method 1
an absorbance measurement unit configured to measure absorbance in a plurality of different wavelength regions for a mixed liquid
Data Source
AI summary
To improve measurement accuracy.A concentration measurement device includes: an absorbance measurement unit configured to measure absorbance in a plurality of different wavelength regions for a mixed liquid in which different liquids are mixed; and a control unit configured to correct a concentration of at least one liquid on the basis of absorbance in the plurality of different wavelength regions. The absorbance measurement unit measures absorbance in two wavelength regions. The absorbance measurement unit measures absorbance in a spectrum. Particles contained in one of the different liquids are different from particles contained in another of the different liquids. Each of the different liquids is a suspension or a solution. The mixed liquid includes a white blood cell suspension and a red blood cell suspension. The control unit calculates a mixing ratio of a liquid mixed in the mixed liquid, from absorbance in the plurality of different wavelength regions. The control unit corrects the absorbance on the basis of a mixing ratio of a liquid mixed in the mixed liquid.