Multispectral Medium Sensing for Raman Scattering Correction
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Solution Overview
Problem
Raman spectrometers face issues with overall fluorescence and Mie scattering from microorganisms, which interfere with accurate detection and quantification of compounds in reaction media, especially due to changing concentrations and geometries of microorganisms.
Innovation Solution
A facility with separate illumination and excitation paths using visible polychromatic and monochromatic light, combined with visible-band and Raman spectrometers, collects and processes both types of electromagnetic radiation to obtain multispectral information, correcting for Mie scattering and providing reliable measurements.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a Raman spectrometer is used to detect compounds in a reaction medium, then molecular detection capability is provided, but the Raman spectrum is concealed by overall fluorescence and Mie scattering from microorganisms
Solution Approach 1:
The patent segments the detection process into two distinct spectral acquisition paths: a visible-band path for capturing overall medium information (including fluorescence and Mie scattering) and a Raman path for capturing molecular vibrations. By separating these detection functions and processing them independently before integration, the system can identify and correct scattering effects while preserving molecular detection accuracy.
Solution Approach 2:
The visible-band spectrum acts as an intermediary that captures the interfering effects (fluorescence and Mie scattering) separately. This intermediary measurement allows the system to model and subtract the scattering contributions from the Raman spectrum, thereby recovering the true molecular signal without direct contamination.
2Reliability
If conventional Raman spectroscopy is applied to monitor microorganism cultures, then compound quantification is possible, but measurement reliability deteriorates due to changing microorganism concentration and geometry
Solution Approach 1:
The system uses the visible-band spectrum as a feedback signal that continuously monitors changes in the medium's optical properties (fluorescence intensity, Mie scattering characteristics) caused by varying microorganism concentration and geometry. This feedback information is fed into the processing algorithm to dynamically adjust and correct the Raman spectrum interpretation, maintaining measurement reliability despite changing conditions.
Solution Approach 2:
The patent monitors changes in optical parameters (visible-band absorption, scattering coefficients) that correlate with microorganism population dynamics. By tracking these parameter changes and using them to adjust the Raman analysis model, the system adapts to evolving medium conditions while maintaining accurate compound quantification.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The facility enables accurate detection and quantification of medium characteristics by correcting for Mie scattering and accessing microorganism states, allowing reliable monitoring of cultures despite changes in optical behavior.
Implementation Method 1
illumination means that are configured to illuminate the medium with visible polychromatic light
Implementation Method 2
excitation means which are configured to excite the medium with a visible-light or infrared monochromatic radiation
Implementation Method 3
at least one Raman spectrometer, which is connected to said second collection and conveying means, and which is configured to obtain at least one Raman spectrum of the medium
Implementation Method 4
the overall fluorescence of the Raman signal. This overall fluorescence depends on the nature of the compounds present in the medium
Data Source
AI summary
The facility for detecting at least one characteristic parameter of a medium (M), has A DEVICE (3) illuminating the medium (M) with a visible polychromatic light, a device (4) exciting the medium (M) with a visible-light or infrared monochromatic radiation, a first device (5) collecting and conveying at least a first electromagnetic radiation emitted by the medium (M), a second device (6) collecting and conveying at least a second electromagnetic radiation emitted by the medium (M), at least one visible-band spectrometer (7) obtaining at least one visible-band spectrum of the medium (M) from the at least first electromagnetic radiation, at least one Raman spectrometer (8) obtaining at least one Raman spectrum of the medium (M) from the at least second electromagnetic radiation, and a device (9) detecting the at least one characteristic parameter from the at least one visible-band spectrum and the at least one Raman spectrum.