Multi-Stage Colony Coating Structure for High-Pressure Separation
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Solution Overview
Problem
Existing microorganism separation devices for high-pressure environments have low efficiency and are limited to single streaking separation, making it difficult to separate and cultivate single colonies effectively.
Innovation Solution
A single colony multi-stage coating-separating device with vertically stacked culture dishes and a stirring rod system that drives a coating unit to perform rotational movement, allowing for automatic multi-stage coating and separation of microorganisms under high-pressure conditions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If multiple streaking separations are performed manually, then single colony separation efficiency improves, but operation complexity and time consumption increase
Solution Approach 1:
The culture device is divided into multiple culture dishes stacked vertically, with each dish performing a single streaking separation stage. The sample passes through each dish sequentially, achieving multi-stage separation without requiring manual repeated operations. This segmentation transforms a complex multi-step manual process into a simple automated flow.
Solution Approach 2:
The stirring rod automatically performs the coating and streaking actions in each culture dish without manual intervention. The system serves itself by using the rotating stirring rod to distribute the sample uniformly across each culture dish surface, eliminating the need for operators to manually perform repeated streaking operations.
2Area of stationary object
If a single culture dish is used, then device simplicity is maintained, but coating area and single colony separation probability are limited
Solution Approach 1:
The culture dishes are arranged vertically in the axial direction rather than horizontally, utilizing the vertical dimension to expand the total coating area. Multiple culture dishes are stacked one above another, allowing the sample to be coated and separated across multiple levels, significantly increasing the effective separation area without requiring a larger horizontal footprint.
3Manufacturing precision
If the stirring rod rotates the culture dish, then coating uniformity improves, but the culture dish cannot be removed and contamination risk increases
Solution Approach 1:
The stirring rod is equipped with a coating unit at its lower end that applies the coating function locally to the culture dish surface. Instead of rotating the entire culture dish, only the coating action is performed locally by the rotating coating unit, allowing the culture dish to remain stationary and maintain its removability while still achieving uniform coating.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enhances the culturability of marine microorganisms by enabling uniform coating over a large area and efficient separation of single colonies, improving the efficiency of microorganism cultivation.
Implementation Method 1
driving the stirring rod according to a preset rotation speed and a preset time
Implementation Method 2
when the coating unit moves to the separating opening area of a current level, the coating unit loses the support provided by the corresponding culture dish, and automatically falls onto the culture dish of a next level under the action of gravity
Data Source
AI summary
A single colony multi-stage coating-separating device for a high-pressure environment is provided, including a culture device and a liquid injection port installed at the top of an inner wall of the culture device. At least two culture dishes are stacked at intervals in the culture device along an axial direction of the culture device, the center of each culture dish is provided with an avoiding hole, and the avoiding hole radially expands towards an edge of the culture dish to form a fan-shaped separating opening. Adjacent separating openings are staggered in the vertical direction, a stirring rod is arranged to penetrate the central axis of the culture device, a lower end of the stirring rod is sleeved with a coating unit, and the projection of the coating unit on the horizontal plane falls within the projection of the separating openings.