Multiwell Filtration Device for Protein Separation
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Solution Overview
Problem
Current methods for obtaining proteins of interest are labor-intensive and inefficient, involving multiple steps and equipment such as centrifuge tubes, pipette tips, and syringe filters, which are not environmentally friendly and require significant time and resources.
Innovation Solution
A multiwell device comprising an upper receiving plate, a middle filtration plate with a three-layer filter, and a lower collection plate, allowing for efficient filtration and separation of proteins from cell samples, reducing labor and environmental impact by processing larger volumes in a single device.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional methods using centrifuge tubes, pipette tips, and syringe filters are used, then protein separation can be achieved, but the process becomes labor-intensive and time-consuming
Solution Approach 1:
The patent combines multiple separate filtration steps into a single integrated device with multiple filters arranged in series. The device integrates a depth filter, a 0.45-micron filter, and a 0.2-micron filter within one multiwell plate structure, allowing simultaneous filtration of multiple samples through a unified system rather than requiring separate centrifuge tubes, pipette tips, and syringe filters for each sample.
Solution Approach 2:
The multiwell device serves multiple functions within a single platform: it performs sample reception, multi-stage filtration, and protein collection simultaneously. The device can process multiple samples in parallel while incorporating different pore size filters for comprehensive particle removal, replacing the need for multiple specialized tools and manual operations.
2Productivity
If traditional filtration methods are used, then proteins can be separated from cells, but the process requires significant labor and resources
Solution Approach 1:
The patent merges multiple filtration operations into a single device structure where samples flow sequentially through depth filtration, 0.45-micron filtration, and 0.2-micron filtration stages. This consolidation eliminates the need for manual transfer between multiple containers and filters, reducing both labor requirements and the complexity of operational procedures.
3Reliability
If multiple separate filtration steps are used, then thorough particle removal is achieved, but the process becomes complex and resource-intensive
Solution Approach 1:
The patent segments the filtration function into distinct sequential stages within a unified device: a depth filter layer for large particle removal, followed by a 0.45-micron filter for intermediate particles, and finally a 0.2-micron filter for fine particle removal. Each filter type is optimized for its specific function, achieving thorough particle removal while maintaining a manageable device structure through systematic segmentation of the filtration process.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The multiwell device enables a less labor-intensive and faster method for obtaining proteins, processing larger volumes while being more environmentally friendly compared to traditional methods, with effective protein recovery and cell removal, as demonstrated by the separation of proteins from CHO cell cultures.
Implementation Method 1
each comprising a filter for filtering the fluid sample passing through the bottom fluid flow port of a corresponding well in the upper receiving plate
Implementation Method 2
an upper depth filter layer having an average pore size in the range of from about 1 micrometer to about 20 micrometers
Implementation Method 3
a middle layer comprising a microporous membrane having an average pore size in the range of from about 0.4 micrometers to about 0.8 micrometers
Implementation Method 4
the fluid flow port in the middle filtration plate is arranged to allow a filtered fluid sample to pass from a well in the middle filtration plate to a corresponding well in the lower collection plate
Data Source
AI summary
Multiwell devices and methods of filtration using the multiwell devices are disclosed.