Multiwell Plate Lid With Sealing Foil For Contamination Prevention
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Solution Overview
Problem
Current automated systems for nucleic acid isolation and amplification face challenges in preventing contamination between wells during the amplification process, particularly in multiwell plates, where sealing methods like gluing or heat sealing are not always effective.
Innovation Solution
An automated process involving a multiwell plate with a lid and sealing foil, where the lid is lifted, the nucleic acid analyte is transferred, and the foil is heat-sealed to create a sealed environment for amplification, ensuring the analyte is isolated and amplified in the presence of reagents.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If gluing or heat sealing is used to seal the multiwell plate, then the sealing function is achieved, but contamination between wells cannot be effectively prevented
Solution Approach 1:
The sealing system is divided into three distinct components: a lid for covering the plate, a sealing foil for heat sealing to the plate surface, and a frame connecting the two. This segmentation allows each component to perform its specific function optimally - the lid provides mechanical coverage, the sealing foil creates hermetic seals around individual wells, and the frame integrates them together, collectively achieving effective contamination prevention that single-method sealing cannot accomplish
Solution Approach 2:
The sealing system combines multiple materials with different properties: the lid material provides structural integrity and ease of handling, the sealing foil material provides heat-sealing capability and hermetic barrier properties, and the frame material provides structural support and connection functionality. This composite approach leverages the strengths of each material to achieve reliable sealing and contamination prevention
2Ease of operation
If the lid is placed directly on the multiwell plate, then the plate is covered, but the sealing foil cannot effectively seal the wells
Solution Approach 1:
The sealing foil is pre-attached to the frame in a ready-to-seal configuration before the lid is placed on the plate. This preliminary preparation allows the sealing foil to be positioned correctly and sealed to the plate surface in advance, ensuring effective well sealing is established before the lid is installed, thereby achieving both ease of operation and reliable sealing
Solution Approach 2:
The frame acts as an intermediary component between the lid and the sealing foil. It provides a structural platform that holds the sealing foil in the correct position and facilitates the heat sealing process, while also connecting to the lid to complete the sealed assembly. This intermediary structure enables the sealing foil to effectively seal the wells while maintaining ease of lid placement
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method effectively prevents contamination and ensures efficient amplification of nucleic acid analytes by creating a sealed environment within the multiwell plate, enhancing the reliability and accuracy of nucleic acid analysis.
Implementation Method 1
heat sealing said second multiwell plate with said sealing foil
Data Source
AI summary
The present invention relates to a multiwell plate for amplification and a lid with a foil for sealing the multiwell plate, wherein two positions of said lid on said plate exist, one position for storage, one position for sealing of the foil to the plate. The invention also relates to a method for.