Mutant CO Dehydrogenase for Oxygen-Resistant CO Oxidation
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Solution Overview
Problem
Existing carbon monoxide dehydrogenases (CO dehydrogenases) are vulnerable to oxygen, leading to rapid decrease in enzymatic activity, making it difficult to effectively convert carbon monoxide into carbon dioxide in the presence of oxygen-containing waste gases.
Innovation Solution
Development of a CO dehydrogenase with enhanced oxygen resistance and enzyme activity, achieved through site-directed mutagenesis and recombinant vector expression, allowing the enzyme to maintain activity even in the presence of oxygen.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Power
If conventional CO dehydrogenase is used, then high unit activity is achieved, but enzymatic activity rapidly decreases due to vulnerability to oxygen molecules
Solution Approach 1:
The patent applies parameter changes by modifying the amino acid sequence of CO dehydrogenase through site-directed mutagenesis. Specifically, it introduces mutations at positions 82, 559, 565, 578, 580, 586, 593, 597, and 610 to enhance oxygen resistance while maintaining or improving enzymatic activity. This direct modification of molecular parameters resolves the contradiction between high unit activity and oxygen vulnerability.
2Quantity of substance
If existing adsorbents are used in cigarette filters, then general adsorption is achieved, but carbon monoxide is not effectively removed due to lack of specificity
Solution Approach 1:
The patent applies local quality by introducing specific amino acid substitutions at targeted positions within the CO dehydrogenase structure. These localized changes create specific binding sites or structural features that enhance the enzyme's ability to recognize and process carbon monoxide selectively, thereby improving CO removal efficiency while maintaining overall adsorption capacity.
Solution Approach 2:
The patent modifies the enzyme's molecular parameters through amino acid substitutions to enhance its specificity for carbon monoxide. By changing the chemical properties of specific residues, the enzyme gains improved selectivity for CO over other gases, directly addressing the harm of insufficient CO removal in cigarette filters.
3Productivity
If CO dehydrogenase is exposed to oxygen-containing waste gas, then CO oxidation is attempted, but enzymatic activity rapidly decreases preventing successful conversion
Solution Approach 1:
The patent applies preliminary action by pre-modifying the CO dehydrogenase enzyme through site-directed mutagenesis before exposing it to oxygen-containing waste gas. The amino acid substitutions are introduced in advance to create an oxygen-resistant enzyme variant that can maintain its activity when subsequently exposed to oxygen, thereby enabling successful CO conversion in realistic waste gas conditions.
Solution Approach 2:
The patent changes the molecular parameters of the enzyme to improve its stability under oxygen exposure. By substituting specific amino acids, the enzyme's structural and functional parameters are optimized to resist oxygen-induced inactivation, thereby maintaining productivity in oxygen-containing environments.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The modified CO dehydrogenase can efficiently convert carbon monoxide into carbon dioxide at room temperature and pressure, even in the presence of oxygen, effectively removing carbon monoxide from various sources including industrial emissions and cigarette smoke.
Implementation Method 1
carbon monoxide dehydrogenase... is an enzyme capable of converting highly toxic carbon monoxide gas into carbon dioxide by oxidizing carbon monoxide at room temperature and pressure
Implementation Method 2
carbon monoxide dehydrogenase (CO dehydrogenase: CODH) is one of metabolic enzymes in microorganisms
Data Source
AI summary
Provided is a carbon monoxide (CO) dehydrogenase with increased oxygen resistance and/or enzyme activity, specifically, a mutant CO dehydrogenase with increased oxygen resistance and/or enzyme activity by mutating amino acid residues. The CO dehydrogenase may detoxify toxic carbon monoxide at room temperature and pressure by easily oxidizing carbon monoxide and converting the same into carbon dioxide, and may effectively oxidize carbon monoxide even in gas including oxygen. Furthermore, since it is possible to remove carbon monoxide, which is emitted in large quantities in industries such as petrochemical and steel industries, cigarette burning, household cooking, various boilers, and combustion, through cigarette filters, air purifiers, intake filters in household cooking equipment, gas boilers, etc. the CO dehydrogenase may be utilized in various ways.