Mutant Collagenase ColH-FM for Non-Invasive Fat Reduction
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Solution Overview
Problem
Current methods for fat reduction, such as liposuction, are invasive and carry significant side effects, while cellulite creams lack effectiveness, and scar reduction products take too long to show results due to the absence of targeted active ingredients like collagenase.
Innovation Solution
A modified mutant collagenase (ColH-FM) with a specific peptide motif is used to target and break down adipose tissue and collagen, allowing for non-invasive fat reduction and scar minimization through injection or topical application.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If liposuction is used to remove excess fat mechanically, then fat reduction is achieved, but it causes infection, bruises, contour deformity, and mechanical damage to tissue
Solution Approach 1:
The patent replaces the mechanical vacuum suction system of liposuction with a biochemical system using mutant collagenase enzymes. These enzymes specifically target and break down adipose tissue through biochemical reactions rather than mechanical force, eliminating the need for physical suction and associated mechanical trauma.
Solution Approach 2:
The patent uses genetically modified collagenase with altered parameters - specifically mutated amino acid sequences that enhance specificity for adipose tissue while reducing non-specific proteolytic activity. This parameter modification allows selective fat breakdown without the harmful effects of traditional mechanical methods.
2Object-affected harmful factors
If cellulite cream is applied to improve appearance, then it is non-invasive, but it only temporarily shrinks fat cells without eliminating them
Solution Approach 1:
The patent introduces mutant collagenase as an intermediary substance that mediates between the non-invasive application method and the effective fat elimination goal. The enzyme acts as a biochemical agent that can be applied topically or by injection, providing intermediate steps that lead to complete fat cell breakdown without mechanical invasion.
3Reliability
If traditional scar reduction products are used, then they are safe, but they take over 3 months to show results due to lack of targeted active ingredients
Solution Approach 1:
The patent extracts and isolates the specific enzymatic activity needed for scar reduction - mutant collagenase with enhanced collagen-digesting capability. By taking out this specific active ingredient and concentrating it in the formulation, the product achieves rapid scar breakdown without compromising safety, eliminating the need for long-term use of ineffective multi-ingredient products.
4Productivity
If wild type collagenase is used for fat reduction, then it breaks down adipose tissue, but it also causes significant bleeding and damage to surrounding tissue
Solution Approach 1:
The patent creates localized quality in the collagenase molecule by introducing site-specific mutations. These mutations modify the enzyme's properties locally at specific amino acid positions, making it selectively active against adipose tissue while reducing its harmful effects on blood vessels and surrounding healthy tissue. The mutant enzyme has altered local characteristics that provide specificity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The mutant collagenase effectively reduces fat and scars by breaking down adipose tissue and collagen, offering a gentle, precise, and minimally invasive alternative to traditional methods with reduced side effects and faster results.
Implementation Method 1
A modified mutant collagenase (ColH-FM) with a specific peptide motif is used to target and break down adipose tissue and collagen
Data Source
AI summary
A Clostridium histolyticum collagenase ColH (Glu451Asp) melts adipose tissue when injected into selected regions of the body. This protein product melts fat pads effectively in fat rat experiments, with very little side effects. Very little hemorrhage was observed. We also invent a new version of ColH mutant by linking a peptide motif CKGGRAKDC-G (varying from 2 to 6 Gs) (SEQ ID NO: 2) in front of ColH (Glu451Asp) (called topical ColH-FM), which can target to white fat vasculature. By combining with novel transdermal technology (such as Hydroxysome technology), we develop a topical protein cream that can melt fat. This product can be used as cellulite cream and for chemical liposuction. This topical ColH-FM can also be injected into adipose tissue as a replacement for liposuction or as an adjunct method with liposuction. Since raise scar is formed by overgrowth of collagen, our topical ColH-FM cream is shown to have application in scar reduction.