Mutant Yeast Strain Ethanol Productivity via Gene Substitution
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Solution Overview
Problem
Yeast strains with xylose-metabolizing ability have insufficient ethanol productivity during fermentation, limiting the efficiency of ethanol production from xylose-containing media.
Innovation Solution
Identification and introduction of specific mutant genes, such as CDC123, SUI3, and FPK1, which encode proteins with amino acid substitutions that enhance ethanol fermentation performance in yeast strains, allowing for improved xylose metabolism and ethanol production.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If yeast strains with xylose-metabolizing ability are used, then xylose utilization capability is improved, but ethanol productivity remains insufficient
Solution Approach 1:
The patent applies parameter changes by introducing specific amino acid substitutions at critical positions in proteins (CDC123: L324C, FPK1: G704E, SUI3: S112T) to modify protein function and interaction parameters. These point mutations alter the biochemical parameters of the yeast strain, enabling improved ethanol productivity while maintaining xylose-metabolizing capability through precise molecular modifications rather than complete pathway redesign
2Ease of manufacture
If conventional yeast strains are used for ethanol fermentation, then fermentation process is simple, but ethanol production efficiency from xylose is low
Solution Approach 1:
The patent applies local quality by making targeted modifications at specific locations within the yeast genome rather than comprehensive redesign. The amino acid substitutions are localized to specific positions in CDC123, FPK1, and SUI3 proteins, where they exert discrete functional effects. This localized approach maintains the overall simplicity of the fermentation process while locally improving ethanol production efficiency from xylose through precise molecular changes
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The mutant yeast strains exhibit enhanced ethanol fermentation ability, leading to improved ethanol productivity when cultured in xylose-containing media, with specific mutations like L324C in CDC123, G704E in FPK1, and S112T in SUI3 significantly increasing fermentation efficiency.
Implementation Method 1
a mutant gene associated with improvement in ethanol productivity in ethanol fermentation performed with a yeast strain or so on having xylose-metabolizing ability
Data Source
AI summary
This invention is intended to improve the ethanol fermentation ability of a yeast strain having xylose-metabolizing ability with the use of a mutant gene encoding a mutant protein comprising a consensus sequence comprising a substitution of amino acid in the 30th position in SEQ ID NO: 1, amino acid in the 43rd position in SEQ ID NO: 4, and amino acid in the 31st position in SEQ ID NO: 7 with other amino acid residues.