Mutated Callose Synthase Polypeptide for Enhanced Biosynthesis
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Solution Overview
Problem
Current methods for enhancing callose biosynthesis and accumulation in plants are poorly characterized and inefficient, with limited increases in callose biosynthesis and accumulation achieved through overexpression of wild-type callose synthases.
Innovation Solution
Development of novel polypeptides with specific mutations in conserved amino acid residues, such as R84, R1926, and P189, which enhance callose biosynthesis and accumulation, along with associated polynucleotides, expression cassettes, vectors, and transgenic plants to modify plant cell walls and improve pathogen resistance.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If wild-type callose synthase is overexpressed, then callose biosynthesis is enhanced, but the increase in callose accumulation is limited
Solution Approach 1:
The patent applies parameter changes by mutating specific amino acid residues (R84, R1926, P189) in the callose synthase protein sequence to alter its catalytic activity and substrate binding properties, thereby dramatically enhancing callose accumulation beyond what is achieved through overexpression of wild-type enzyme alone
Solution Approach 2:
The patent creates a composite functional system by combining mutated callose synthase polypeptides with specific polynucleotide sequences, expression cassettes, and vectors to achieve synergistic enhancement of callose biosynthesis and accumulation in transgenic plants
2Reliability
If callose accumulation is enhanced, then pathogen resistance is improved, but cell-to-cell signaling is affected
Solution Approach 1:
The patent applies local quality by enabling targeted enhancement of callose accumulation at specific locations such as plasmodesmata and cell walls through spatially controlled expression of mutated callose synthase, thereby providing localized pathogen resistance while minimizing disruption to overall cell signaling pathways
Data Source
AI summary
The present invention relates to a polypeptide, or a fragment thereof, capable of enhancing callose biosynthesis and/or accumulation, wherein at least one of the conserved amino acid residues selected from the group consisting of residue corresponding to R84 of SEQ ID NO: 1, residue corresponding to R1926 or SEQ ID NO: 1 and residue corresponding to P189 of SEQ ID NO: 1, of the polypeptide or a fragment thereof, is modified by a mutation selected from the group consisting of substitution and deletion.