Mycotoxin Analysis Method Using Dual Detectors
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Solution Overview
Problem
Current analysis methods for mycotoxins in food and drink require multiple analyses under different conditions, making it time-consuming to check the presence or absence of multiple components in a liquid sample.
Innovation Solution
An analysis method using a high-performance liquid chromatograph with a separation step, detection by at least two detectors (including a fluorescence detector and a photodiode array detector), and identification based on detection signals from these detectors, allowing for simultaneous identification of total aflatoxin and deoxynivalenol, with a mobile phase of a mixed buffer and organic solvent, and adjustable solvent mixing ratio and fluorescence wavelengths.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If separate analysis methods are used for each mycotoxin component under different conditions, then identification and quantification accuracy is improved, but analysis time and operational burden increase
Solution Approach 1:
The patent combines multiple analysis methods for different mycotoxin components into a single integrated analysis system. By using a high-performance liquid chromatograph equipped with multiple detectors (fluorescence detector, ultraviolet spectrophotometer detector, and mass spectrometer) that can operate simultaneously under different detection conditions, the system achieves both accurate identification of various mycotoxins and reduced analysis time.
Solution Approach 2:
The analysis system is designed with multi-functional capability to handle different mycotoxin components (aflatoxins B1, B2, G1, G2, and M1) using a single chromatograph. The system can switch between different detection modes and conditions within one analysis run, making it universally applicable to multiple mycotoxin types without requiring separate dedicated analysis procedures for each component.
2Loss of information
If quantitative analysis is performed for each mycotoxin component, then detailed compositional information is obtained, but the complexity and duration of the analysis process increases
Solution Approach 1:
The patent segments the detection process for different mycotoxin components by assigning specific detectors to specific components based on their detection characteristics. The fluorescence detector is optimized for aflatoxins, the ultraviolet spectrophotometer detector for deoxynivalenol, and the mass spectrometer provides comprehensive identification for all components. This segmentation allows detailed compositional information to be obtained while managing analysis complexity through specialized detection pathways.
Solution Approach 2:
The high-performance liquid chromatograph serves as an intermediary system that separates and directs different mycotoxin components to appropriate detectors. The chromatographic separation process acts as a mediator that divides the complex mixture into individual components, which are then detected by the most suitable detector for each component, thereby obtaining detailed compositional information without requiring direct complex analysis of the entire mixture.
3Adaptability or versatility
If multiple analyses are performed by changing conditions for each component, then comprehensive detection coverage is achieved, but operational efficiency decreases
Solution Approach 1:
The patent implements continuous useful action by performing detection of multiple mycotoxin components simultaneously in a single uninterrupted analysis run. The chromatograph operates continuously with multiple detectors working in parallel, eliminating the need to stop and change conditions between analyses for different components. This continuous operation maintains comprehensive detection coverage while significantly improving operational efficiency.
Solution Approach 2:
The system employs periodic action through programmed switching of detection wavelengths and conditions during the analysis run. The chromatograph automatically adjusts detection parameters at specific time intervals to match the elution patterns of different mycotoxin components, enabling comprehensive detection coverage across varying conditions without manual intervention or multiple separate analyses.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid and accurate detection of multiple mycotoxin components in a single analysis, improving efficiency and accuracy compared to traditional methods that require multiple analyses under different conditions.
Implementation Method 1
In the detection step, components separated in the separation step are detected by at least two detectors. The at least two detectors include a fluorescence detector.
Implementation Method 2
deoxynivalenol is identified based on a detection signal from a detector other than the fluorescence detector
Data Source
AI summary
An analysis method for mycotoxins including a separation step, a detection step, and an identification step. In the separation step, each component contained in a liquid sample is separated in a column In the detection step, components separated in the separation step are detected by a PDA and a fluorescence detector. In the identification step, total aflatoxin is identified based on a detection signal from the fluorescence detector, and deoxynivalenol is identified based on a detection signal from the PDA.