Dual-Layer Extraction Kit for Simultaneous Mycotoxin Detection
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Solution Overview
Problem
Current extraction kits for detecting mycotoxin residues in agricultural products are diverse and expensive, requiring low temperature storage and different methods for various types of mycotoxins, making them inefficient and costly for simultaneous detection.
Innovation Solution
A kit comprising a pipe with two powder mixture layers, one containing cation exchange resin, C18, and diatomaceous earth, and the other with PSA, anhydrous magnesium sulfate, activated carbon, PLS, diatomaceous earth, and C18, which allows for the simultaneous extraction of 11 types of mycotoxin residues by flowing a sample solution through the layers.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If multiple different extraction kits and methods are used to detect different types of mycotoxin residues, then detection coverage is improved, but device complexity and operation complexity increase
Solution Approach 1:
The patent combines multiple extraction methods into a single integrated extraction kit that can simultaneously extract multiple types of mycotoxins (aflatoxins, deoxynivalenol, fumonisins, HT-2 toxin, ochratoxin A, T-2 toxin, and zearalenone) using one unified procedure, thereby reducing the number of separate kits needed
Solution Approach 2:
The extraction kit is designed with universal applicability to detect multiple mycotoxin types simultaneously through a single extraction process, making one kit serve multiple detection purposes that previously required separate specialized kits
2Measurement precision
If enzyme immunoaffinity extraction kits are used for mycotoxin detection, then detection accuracy is improved, but storage requirements become more stringent and cost increases
Solution Approach 1:
The patent employs disposable extraction cartridges with pre-packed sorbent materials that do not require low-temperature storage, replacing the need for expensive enzyme immunoaffinity extraction kits that must be stored under stringent temperature conditions
Solution Approach 2:
The extraction mechanism transitions from enzyme-based immunoaffinity extraction requiring cold storage to a sorbent-based physical/chemical extraction method that operates at ambient temperatures, changing the fundamental extraction parameter from biochemical to physicochemical
3Adaptability or versatility
If traditional extraction methods are used for multiple mycotoxin detection, then detection comprehensiveness is improved, but solvent consumption and operation time increase
Solution Approach 1:
The method combines multiple extraction procedures into a single streamlined operation where one extraction process simultaneously extracts multiple mycotoxin types, eliminating the need for separate extractions and reducing total solvent consumption
Solution Approach 2:
The extraction process operates continuously through a single pass through the extraction cartridge, maintaining constant flow and extraction efficiency without interruption, thereby reducing both time and solvent usage compared to sequential extraction methods
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The kit reduces solvent consumption by 35-90% and extraction time by 80-95%, enabling efficient simultaneous detection of multiple mycotoxin residues without the need for different extraction kits and methods.
Implementation Method 1
The first powder mixture layer contains cation exchange resin powder, C18 and diatomaceous earth powder
Implementation Method 2
The first powder mixture layer contains cation exchange resin powder
Implementation Method 3
The second powder mixture layer contains PSA powder, anhydrous magnesium sulfate powder, activated carbon, PLS powder, diatomaceous earth powder and C18 powder
Implementation Method 4
The second powder mixture layer is located below the first powder mixture layer and above the output port
Data Source
AI summary
A kit for extracting mycotoxin residues in agricultural products according to the present disclosure includes a pipe, a first powder mixture layer and a second powder mixture layer. The pipe has an output port at the bottom thereof and an input port at the top thereof for inputting a sample solution. The first powder mixture layer is in the form of powder and filled in the pipe. The first powder mixture layer contains cation exchange resin powder, C18 and diatomaceous earth powder. The second powder mixture layer is in the form of powder and filled in the pipe. The second powder mixture layer is located below the first powder mixture layer and above the output port. The second powder mixture layer contains PSA powder, anhydrous magnesium sulfate powder, activated carbon, PLS powder, diatomaceous earth powder and C18 powder. The present disclosure further provides a method of obtaining a primary test liquid from agricultural products using the above kit.