Myocardial Cell Assay Priming for Stable Heart Rate Drug Evaluation
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Solution Overview
Problem
Existing methods for evaluating the effects of muscarinic receptor agonists and adrenergic receptor inhibitors on myocardial cells are inefficient and unable to assess muscarinic receptor inhibitors effectively.
Innovation Solution
A drug evaluation method involving treating myocardial cells with a culture medium containing physiologically active substances of the sympathetic and parasympathetic nervous systems, such as adrenergic receptor agonists and muscarinic receptor agonists, followed by drug exposure, allowing for high-throughput evaluation of drug effects on heart rate.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If myocardial cells are used for drug evaluation without pre-treatment with physiologically active substances, then the evaluation process is simple, but the ability to evaluate muscarinic receptor agonists and adrenergic receptor inhibitors is insufficient
Solution Approach 1:
The patent applies preliminary action by pre-treating myocardial cells with physiologically active substances from the sympathetic and parasympathetic nervous systems before drug exposure. This priming step enables the cells to properly respond to various drug types including muscarinic receptor agonists, adrenergic receptor agonists, and their inhibitors, thereby improving evaluation reliability without requiring complex additional equipment
2Productivity
If conventional evaluation methods are used, then the procedure is straightforward, but high-throughput evaluation of multiple drug types is not achievable
Solution Approach 1:
The patent implements universality by creating a multi-functional evaluation system where pre-treated myocardial cells can assess multiple drug types (muscarinic receptor agonists, adrenergic receptor agonists, and their inhibitors) using the same basic assay platform. This enables high-throughput evaluation while maintaining operational simplicity through a standardized protocol
3Measurement precision
If no pre-treatment with physiologically active substances is applied, then the culture medium composition is simple, but accurate assessment of drug effects on heart rate is not achieved
Solution Approach 1:
The patent applies preliminary action by incorporating physiologically active substances from the sympathetic and parasympathetic nervous systems into the culture medium before drug exposure. This pre-treatment prepares the myocardial cells to accurately reflect drug effects on heart rate, improving measurement precision while adding only moderate complexity to the culture medium composition
Data Source
AI summary
An object of the present invention is to provide a drug evaluation method using myocardial cells, which can stably evaluate an effect of a drug such as a muscarinic receptor agonist, an adrenergic receptor inhibitor or a muscarinic receptor inhibitor on a heart rate; and a reagent and a kit for carrying out the drug evaluation method. According to the present invention, there is provided a drug evaluation method including: treating myocardial cells with a culture medium containing a physiologically active substance of a sympathetic nervous system and/or a physiologically active substance of a parasympathetic nervous system; bringing a drug into contact with the myocardial cells treated as above; and evaluating an effect of the drug on a heart rate of the myocardial cells.