Myocardial Cell Sheet Proportion Optimization
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Solution Overview
Problem
Current methods for repairing injured myocardial tissue using myocardial cells derived from pluripotent stem cells face challenges in optimizing the proportion of myocardial cells in sheet-shaped cell cultures, which affects their functional properties and cytokine production capabilities.
Innovation Solution
A sheet-shaped cell culture is developed with a controlled proportion of myocardial cells derived from pluripotent stem cells, ranging from 50% to 70%, including αSMA positive cells, CD31 positive cells, and TE-7 antigen positive cells, to enhance cytokine production and therapeutic efficacy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If the proportion of myocardial cells in the cell population is increased to enhance therapeutic effect, then the functional properties improve, but the cytokine production capability decreases
Solution Approach 1:
The patent applies parameter changes by optimizing the proportion of myocardial cells in the cell population to a specific range (50-70%). This quantitative parameter adjustment resolves the contradiction by finding the optimal balance point where both functional properties and cytokine production are maximized, rather than simply increasing myocardial cell proportion without limitation.
2Quantity of substance
If the proportion of myocardial cells is controlled within 50% to 70%, then the cytokine producing ability is enhanced, but the purity of myocardial cells is reduced
Solution Approach 1:
The patent deliberately changes the purity parameter from near-100% myocardial cells to a controlled range of 50-70% myocardial cells mixed with other cell types. This parameter transformation reveals that moderate purity with diverse cell types actually enhances cytokine production, resolving the contradiction between purity and functional output.
Solution Approach 2:
The patent creates a composite cell population structure consisting of myocardial cells (50-70%) combined with other cell types (30-50%). This composite approach leverages the synergistic effects between different cell types to maximize cytokine production while maintaining adequate myocardial cell content for therapeutic function.
Data Source
AI summary
A sheet-shaped cell culture is disclosed that includes myocardial cells derived from pluripotent stem cells and has excellent functional properties. A sheet-shaped cell culture obtained by culturing a cell population that includes myocardial cells derived from pluripotent stem cells, wherein the proportion of the number of the myocardial cells derived from pluripotent stem cells to the total number of cells in the cell population is 50% to 70%; a method of producing the sheet-shaped cell culture; a composition including the sheet-shaped cell culture; and a method of treating a disease using the sheet-shaped cell culture or the composition.