Multi-Marker Myocardial Infarction Detection Kit
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Solution Overview
Problem
Current diagnostic kits for myocardial infarction lack sensitivity and specificity, leading to delayed or misdiagnosis, especially in the early stages, as they often rely on single markers or combinations that are not effective for rapid detection.
Innovation Solution
A kit that simultaneously detects three markers - human myeloperoxidase (MPO), heart-fatty acid binding protein (FABP3), and cardiac troponin I (cTnI) using a colloidal gold or fluorescence microparticles immunoassay, with specific antibodies and paired antibodies coated on a chromatographic membrane for enhanced sensitivity and specificity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If single cardiac markers are used for detection, then the device complexity is low, but the measurement precision and reliability are insufficient leading to delayed diagnosis
Solution Approach 1:
The patent combines three different cardiac markers (MPO, FABP3, and cTnI) into a single rapid detection kit. The kit uses a chromatographic membrane with multiple test lines, each coated with specific antibodies for different markers. Sample liquid containing these markers passes through the membrane, and colloidal gold-labeled antibodies detect each marker simultaneously, enabling comprehensive cardiac injury assessment in one test.
Solution Approach 2:
The rapid detection kit serves multiple diagnostic functions simultaneously. It can detect MPO for early inflammatory response, FABP3 for early myocardial injury, and cTnI for established myocardial infarction. This multi-functional approach allows the single kit to provide comprehensive cardiac injury evaluation across different disease stages, replacing the need for separate single-marker tests.
2Loss of time
If early stage markers like MPO and FABP3 are detected, then the diagnosis timing is improved, but the measurement precision requirements increase
Solution Approach 1:
The patent optimizes detection parameters including colloidal gold particle size (15-50 nm), antibody concentrations, and buffer compositions to enhance sensitivity for early markers. The chromogenic substrates and reaction conditions are carefully adjusted to detect low concentrations of MPO and FABP3 that appear in early myocardial injury, achieving high sensitivity without compromising detection speed.
Solution Approach 2:
The patent uses colloidal gold particles as intermediaries to amplify the detection signal. The colloidal gold-labeled antibodies bind to the target markers (MPO, FABP3, cTnI) and produce visible color changes, enhancing the detection sensitivity for early-stage markers. This intermediary approach allows reliable detection of low-concentration markers while maintaining rapid test performance.
3Reliability
If multiple markers are detected simultaneously, then the reliability of diagnosis is improved, but the ease of operation may be reduced
Solution Approach 1:
The chromatographic membrane is segmented into multiple test lines, each dedicated to detecting a specific marker (MPO, FABP3, or cTnI). This segmentation allows simultaneous detection of multiple markers in different locations on the same membrane, enabling reliable comprehensive diagnosis while maintaining a simple single-step operation format where sample liquid automatically flows through all test lines.
Solution Approach 2:
The rapid detection kit is designed as a self-service system where the sample liquid automatically flows through the chromatographic membrane via capillary action, and the colloidal gold reactions occur automatically without requiring external power or complex operations. The kit performs multiple marker detections simultaneously through this self-driven process, maintaining ease of operation while achieving reliable multi-marker assessment.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The kit provides early warning and accurate diagnosis of myocardial infarction with high sensitivity and specificity, enabling timely medical intervention and reducing misdiagnosis, allowing for point-of-care testing and dynamic monitoring of myocardial infarction.
Implementation Method 1
a sample pad, a conjugate pad, a chromatographic membrane coated with three test line and a quality control line, and a sample absorption pad
Implementation Method 2
detecting antibodies of the three markers being all marked on the conjugate pad, and the chromatographic membrane having three test lines formed by coating paired antibodies of the three markers respectively, the paired antibodies of the three markers being able to specifically combine with the three markers
Implementation Method 3
A kit that simultaneously detects three markers - human myeloperoxidase (MPO), heart-fatty acid binding protein (FABP3), and cardiac troponin I (cTnI) using a colloidal gold or fluorescence microparticles immunoassay
Implementation Method 4
using a colloidal gold or fluorescence microparticles immunoassay
Data Source
AI summary
Disclosed are a kit for testing myocardial infarction rapidly and a preparation method and use thereof. The kit comprises a strip capable of detecting three markers, namely, human myeloperoxidase (MPO), heart-fatty acid binding protein (FABP3) and cardiac troponin I (cTnI) simultaneously. The strip comprises a sample pad, a conjugate pad, a chromatographic membrane coated with three test lines and a quality control line, and a sample absorption pad. Antibodies of the three markers are all marked on the conjugate pad. The chromatographic membrane has three test lines formed by coating paired antibodies of the three markers respectively, the paired antibodies of the three markers being able to specifically combine with the three markers, respectively. The kit has advantages such as convenient operation, rapid response, high sensitivity and high specificity, point of care test, and economical and practical etc.