Enzymatic N-3 Polyunsaturated Fatty Acid Diacylglycerol Preparation
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Solution Overview
Problem
Current methods for preparing n-3 polyunsaturated fatty acid diacylglycerol, such as high-temperature partial hydrolysis and enzymatic methods, face challenges including high costs, formation of harmful substances, and inefficient reaction processes, limiting their industrial application.
Innovation Solution
A method involving high-pressure homogenization of oil and water mixtures rich in n-3 polyunsaturated fatty acids before enzymatic hydrolysis with a lipase, such as Candida cylindracea-derived lipase, to enhance catalytic efficiency and reduce reaction time and lipase usage.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If high-temperature partial hydrolysis is used to prepare diacylglycerol oil, then the production cost is reduced and the process is simplified, but harmful substances such as glycidyl esters and chloropropanol esters are formed
Solution Approach 1:
The patent changes the temperature parameter from high-temperature treatment to mild temperature (30-50°C) enzymatic hydrolysis, and changes the chemical environment from harsh chemical catalysts to biological enzymes, thereby eliminating harmful substance formation while maintaining production feasibility
Solution Approach 2:
The patent replaces the chemical hydrolysis mechanism with enzymatic hydrolysis mechanism, using lipase enzymes to catalyze the reaction instead of chemical catalysts and high-temperature treatment, thereby avoiding the formation of harmful substances while keeping the process economically viable
2Object-generated harmful factors
If enzymatic method with lipase is used to prepare diacylglycerol oil, then harmful substances content is reduced and reaction conditions are mild, but the production cost increases due to high lipase cost
Solution Approach 1:
The patent optimizes physical parameters including homogenization pressure (5-50 MPa), temperature (30-50°C), and reaction time to maximize enzyme efficiency, thereby reducing the amount of lipase needed and lowering production cost while maintaining the benefits of mild reaction conditions
Solution Approach 2:
The patent applies preliminary high-pressure homogenization treatment to the oil before enzymatic hydrolysis, which pre-processes the substrate to improve enzyme accessibility and catalytic efficiency, thereby reducing the quantity of expensive lipase required
3Device complexity
If traditional enzymatic hydrolysis is used without homogenization, then the process is simple, but the reaction efficiency is low and hydrolysis time is extended
Solution Approach 1:
The patent applies preliminary high-pressure homogenization treatment to break down the oil structure and increase surface area before enzymatic hydrolysis, which significantly improves enzyme-substrate contact and reaction efficiency without adding complex process steps
Solution Approach 2:
The patent introduces homogenization pressure as a new controllable parameter (5-50 MPa) to optimize the physical state of the oil substrate, thereby enhancing reaction efficiency and reducing hydrolysis time while maintaining process simplicity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly improves hydrolysis efficiency, reduces the amount and cost of lipase required, and increases diacylglycerol content, making the process more cost-effective and suitable for industrial production.
Implementation Method 1
employing selective enzymatic hydrolysis method to prepare diacylglycerol
Implementation Method 2
adding a lipase for undergoing an enzymatic reaction
Implementation Method 3
the reaction raw materials are homogenized to improve the catalytic efficiency of lipase in the hydrolysis mixed reaction system
Implementation Method 4
adding an oil and water to a reactor for a high-pressure homogenization
Data Source
AI summary
A method for enzymatically preparing a n-3 polyunsaturated fatty acid diacylglycerol using oil rich in n-3 polyunsaturated fatty acid as raw material to prepare diacylglycerol oil with selective enzymatic hydrolysis. Before the enzymatic reaction, the raw materials are homogenized to improve the catalytic efficiency of lipase in the hydrolysis and achieve the purpose of reducing the reaction time and the addition amount of lipase. As a result, the cost of industrial diacylglycerol preparation is reduced, ultimately reducing the cost of industrial diacylglycerol preparation. Therefore, the present invention provides an effective, mild and low-cost enzymatic method for preparing diacylglycerol rich in n-3 polyunsaturated fatty acids, which has a promising potential for industrial application.