NADH Fluorescence Imaging for Ischemia Detection
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Solution Overview
Problem
Current methods for determining tissue ischemia are invasive, costly, and unable to provide real-time, non-invasive assessments.
Innovation Solution
The method involves exciting NADH fluorescence and processing the fluorescence intensity values over time to assess ischemic damage, using techniques such as standard deviation calculation, differential equation modeling, and neural network classification.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional imaging modalities (CT, MRI, Ultrasound) are used to visualize ischemia, then areas with reduced blood flow can be identified, but the methods are expensive and cannot provide real-time assessment
Solution Approach 1:
The patent replaces complex mechanical imaging systems (CT, MRI, Ultrasound) with a fluorescence-based optical method. By using NADH fluorescence imaging, the system achieves real-time ischemia detection without requiring expensive and complex imaging equipment, thus substituting mechanical systems with a simpler optical approach.
Solution Approach 2:
The patent monitors changes in NADH fluorescence intensity as a parameter to detect ischemia. By tracking the temporal dynamics of fluorescence intensity changes rather than using static anatomical imaging, the system can detect metabolic changes associated with ischemia in real-time, providing a different parameter-based approach to the same diagnostic goal.
2Measurement precision
If blood tests are administered to check for substances released when organs are damaged, then cardiac troponin levels can be measured, but the method is invasive and cannot provide real-time monitoring
Solution Approach 1:
The patent replaces invasive mechanical procedures (blood draws, biopsies) with non-invasive optical imaging. By using fluorescence imaging to detect NADH changes, the system eliminates the need for invasive sampling while maintaining diagnostic accuracy for detecting tissue damage and ischemia.
Solution Approach 2:
The patent uses NADH fluorescence as an intermediary marker to detect tissue ischemia and damage. Instead of directly measuring tissue properties or requiring invasive access, the system uses the fluorescent properties of NADH as a mediator that can be detected externally, providing indirect but accurate information about tissue state without invasion.
3Measurement precision
If tissue biopsy is undertaken to determine ischemia, then direct tissue assessment can be performed, but the method is invasive and cannot provide real-time monitoring
Solution Approach 1:
The patent replaces invasive mechanical tissue sampling (biopsy) with non-invasive optical imaging. By using fluorescence microscopy or imaging to detect NADH distribution and intensity, the system achieves direct tissue assessment without physical removal or disruption of tissue, eliminating invasiveness while maintaining diagnostic capability.
Solution Approach 2:
The patent uses NADH fluorescence signal as an intermediary to assess tissue ischemia. Instead of requiring direct physical contact with or removal of tissue, the system detects the fluorescent properties of NADH within the tissue, providing indirect measurement that avoids invasion while maintaining accuracy in assessing tissue metabolic state.
4Measurement precision
If functional tests are administered to check organ performance, then the extent of ischemic impact can be determined, but the methods are time-consuming and cannot provide real-time assessment
Solution Approach 1:
The patent enables continuous real-time monitoring of NADH fluorescence intensity to track ischemia progression dynamically. By continuously capturing fluorescence images over time, the system provides uninterrupted assessment of tissue metabolic state, eliminating the time delays inherent in discrete functional tests and enabling immediate detection of ischemic changes.
Solution Approach 2:
The patent uses periodic fluorescence imaging to monitor NADH dynamics over time. By capturing images at regular intervals, the system tracks the temporal evolution of ischemia, providing time-resolved information that is much faster than conventional functional tests while maintaining accurate evaluation of ischemic impact.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for inexpensive, non-invasive, and real-time monitoring of ischemic damage, providing accurate maps of ischemic injuries and enabling dynamic tracking of ischemia progression.
Implementation Method 1
excitation of NADH fluorescence and its registration... under the influence of exciting radiation with a wavelength of 365 nm... emission of blue light with a wavelength of 460 nm
Data Source
AI summary
The invention relates to medicine, particularly clinical medicine, and can be used in medical diagnostics during surgical procedures on organs and tissues or when preserving an organ to assess its functional state and identify functional disorders. The method and the device implementing it both provide an evaluation by a non-invasive method. In some embodiments, the evaluated organ is affected by NADH-exciting radiation with wavelengths in the near ultraviolet range or the visible spectrum, followed by registration of the returned fluorescence, conversion of fluorescent data into digital form, and obtaining an assessment of ischemic damage to the organ tissue.