NADPH Purification via Anion Exchange Resin and Microfiltration
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Solution Overview
Problem
Current methods for purifying NADPH products from biocatalysis, such as liquid chromatography and existing ion exchange resin methods, face challenges with low yield and purity, high costs, and the introduction of impurities due to the use of organic solvents and ion-pairing agents.
Innovation Solution
A novel ion exchange resin method involving pretreatment with a microfiltration membrane, loading onto an anion exchange resin column, elution of cations and impurities using specific sodium chloride and ethanol solutions, and regeneration of the resin to achieve high purity and yield of NADPH, utilizing a macroporous styrenic quaternary ammonium type I strong basic anion exchange resin.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If liquid chromatography is used for purification, then purity is improved, but cost increases and new impurities are introduced due to ion-pairing agents and organic reagents
Solution Approach 1:
The invention extracts and removes the harmful ion-pairing agents and organic reagents from the purification process by using alternative ion exchange resin methods that achieve high purity without introducing new impurities
Solution Approach 2:
The invention uses disposable ion exchange resin columns that can be discarded after single use, eliminating the need for expensive and complex liquid chromatography systems while avoiding contamination from reusable components
2Ease of manufacture
If ion exchange resin method is used for purification, then cost is reduced, but purity and yield are limited to about 95% and 60% respectively
Solution Approach 1:
The invention changes the parameters of the ion exchange resin method by optimizing the resin type, elution conditions, and process parameters to achieve purity of 95% or more and yield of 75% or more, significantly improving upon conventional limitations
Solution Approach 2:
The invention uses composite ion exchange resin materials that combine multiple functional groups and properties to simultaneously achieve high purity, high yield, and cost-effectiveness that neither simple resins nor liquid chromatography can provide alone
3Productivity
If conventional ion exchange resin method is used, then production capacity is limited, but equipment complexity is reduced
Solution Approach 1:
The invention performs preliminary concentration and preparation of the crude NADPH product before ion exchange purification, which enables the process to handle larger volumes and achieve higher production capacity without requiring proportionally larger or more complex equipment
Solution Approach 2:
The invention introduces dynamic optimization of flow rates, elution speeds, and process parameters to maximize the throughput and productivity of relatively simple ion exchange equipment, achieving high production capacity without increasing equipment complexity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves a yield of up to 85% and purity of 98% or higher, is environmentally friendly, and reduces production costs, making it suitable for industrial-scale NADPH purification.
Implementation Method 1
A crude NADPH product prepared by biocatalysis is filtered through a microfiltration membrane that is a hollow fiber membrane having a pore size of 0.1-0.5 μm
Implementation Method 2
The microfiltered crude NADPH product obtained in Step A is loaded onto an anion exchange resin column
Data Source
AI summary
The present invention relates to a process for purifying crude NADPH product prepared by biocatalysis. The objective of the present invention is to solve the technical problems of low yield and low purity of the purified product in the existing ion exchange resin method. The present invention comprises sequentially the following steps: pretreatment, loading onto an ion column, elution of cations, pre-elution of impurities etc. The yield of the purification process disclosed in the present invention can be up to 85% or higher and the purified NADPH has a purity of up to 98% or higher.