Nano-Coated Bacterial Agent for Leaf Adhesion and Rainfall Resistance
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Solution Overview
Problem
Current foliar microbial inoculation systems face challenges with bacterial agents having insufficient adhesion to leaf surfaces, short residence times, and poor survival and colonization rates due to hydrophobicity and environmental stresses, leading to reduced effectiveness in promoting plant growth.
Innovation Solution
A nano-coated growth-promoting bacterial agent is developed using a plant-derived polyphenol and soluble multivalent metal ion self-assembly on the bacterial surface, forming a nano-coating that enhances adhesion and survival in the phyllosphere, protected from environmental stresses.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of moving object
If traditional bacterial solution formulations are used for foliar spraying, then the application process is simple, but the effective residence time on crop leaves is insufficient due to drifting, jumping, rolling, rainfall erosion, and decomposition
Solution Approach 1:
The patent introduces an adhesive agent as an intermediary substance that mediates between the bacterial suspension and the leaf surface. This adhesive agent forms a protective film that anchors bacteria to the leaf surface, preventing drifting, jumping, rolling, and rainfall erosion, thereby significantly extending the effective residence time of bacteria on crop leaves without overly complicating the formulation process
Solution Approach 2:
The patent employs a thin film formed by the adhesive agent that coats the bacterial suspension and adheres to the leaf surface. This flexible film protects the bacteria from environmental stresses including rainfall erosion, ultraviolet radiation, and desiccation, while maintaining the simplicity of spray application
2Reliability
If growth-promoting bacteria are applied to foliar surfaces, then plant growth promotion is achieved, but the strong hydrophobicity of wax layers on leaf surfaces reduces adhesion and colonization
Solution Approach 1:
The adhesive agent serves as a mediator that overcomes the hydrophobic barrier of leaf wax layers. It contains both hydrophilic and hydrophobic components that can interact with both the aqueous bacterial suspension and the hydrophobic wax surface, enabling effective adhesion and colonization
Solution Approach 2:
The patent modifies the surface properties of the bacterial suspension by adding the adhesive agent, which changes the wettability parameters of the formulation. This allows the bacterial suspension to spread and adhere effectively on hydrophobic leaf surfaces, transforming the interaction from repulsive to adhesive
3Reliability
If growth-promoting bacteria are exposed to environmental stresses, then natural selection occurs, but ultraviolet rays, dryness, and rainfall erosion reduce survival and colonization rates
Solution Approach 1:
The adhesive agent provides beforehand cushioning protection to bacteria before they are exposed to environmental stresses. The protective film formed by the adhesive agent cushions bacteria against ultraviolet radiation, prevents desiccation by maintaining moisture, and protects against rainfall erosion, thereby significantly improving survival rates in the phyllosphere environment
Solution Approach 2:
The adhesive agent forms a flexible protective film around and on the bacterial cells that acts as a physical barrier against environmental stresses. This thin film protects bacteria from ultraviolet radiation penetration, prevents water loss during dry periods, and anchors bacteria during rainfall events
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The nano-coating improves leaf surface adhesion, reduces loss under rainfall erosion, and enhances colonization and survival of bacteria, promoting crop growth with improved microbial inoculation efficacy.
Implementation Method 1
a nano-coating formed by a reaction of a plant-derived polyphenol and a soluble multivalent metal ion on the surface of the growth-promoting bacterial agent
Implementation Method 2
the plant-derived polyphenol and the soluble multivalent metal ion self-assemble on the surface of the growth-promoting bacterial agent to form a nano-coating
Implementation Method 3
The nano-coated growth-promoting bacterial agent has strong adhesion to leaf surfaces
Implementation Method 4
The nano-coating formed by the reaction of the plant-derived polyphenol and the soluble multivalent metal ion on the surface of the growth-promoting bacterial agent... can protect the growth-promoting bacterium from stresses such as active oxygen and ultraviolet rays
Data Source
AI summary
The present disclosure belongs to the technical field of microorganisms, and in particular relates to a nano-coated growth-promoting bacterial agent and a composite growth-promoting bacterial agent, preparation methods and use thereof. The nano-coated growth-promoting bacterial agent includes a growth-promoting bacterial agent and a nano-coating formed by self-assembly of a plant-derived polyphenol and a soluble multivalent metal ion on the surface of the growth-promoting bacterial agent. The key to the present disclosure is to employ the plant-derived polyphenol and the soluble multivalent metal ion to self-assemble on the surface of the growth-promoting bacterial agent to form a nano-coating. Based on the interaction of the growth-promoting bacterial agent with the phyllosphere, the leaf face adhesion performance of the growth-promoting bacterial agent is significantly enhanced, the loss rate of the growth-promoting bacterial agent under rainfall erosion is effectively reduced, and the survival rate and colonization of the growth-promoting bacterial agent in the phyllosphere are enhanced.