Nanoaggregate-Embedded Beads for Multiplex Bio-Detection

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Solution Overview

Problem

Fluorescent tags in multiplex bio-detection suffer from photo-bleaching and peak overlapping, limiting their effectiveness in detecting multiple analytes simultaneously.

Innovation Solution

Nanoaggregate-embedded beads are created by adding Raman dye to metal nanoparticles and coating them with silica or metal oxide, then conjugating them with probe molecules for analyte detection, allowing for simultaneous detection of multiple analytes through Raman spectra analysis.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If fluorescent tags are used for multiplex bio-detection, then detection capability is improved, but photo-bleaching and peak overlapping occur

Engineering Contradiction:

Improvemultiplex detection capabilityVSAvoiddetection stability

Core Design Contradiction:

Adaptability or versatilityVSReliability

Solution Approach 1:

The patent replaces the fluorescent detection mechanism with a Raman scattering-based detection mechanism. Instead of using fluorescent tags that suffer from photo-bleaching and peak overlapping, the invention uses Raman dyes embedded in nanoaggregate-embedded beads, which provide stable, non-overlapping spectral signatures for multiplex detection without the drawbacks of fluorescence.

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

2Adaptability or versatility

If fluorescent tags are used for multiplex bio-detection, then detection capability is improved, but peak overlapping occurs

Engineering Contradiction:

Improvemultiplex detection capabilityVSAvoidspectral resolution

Core Design Contradiction:

Adaptability or versatilityVSMeasurement precision

Solution Approach 1:

The patent applies local quality by embedding different Raman dyes with distinct spectral characteristics into specific nanoaggregate-embedded beads. Each bead type contains a unique Raman dye configuration that produces a localized, non-overlapping spectral signature, enabling precise discrimination of multiple analytes simultaneously without spectral interference.

Inventive Principle:

Principle #3Local quality

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This method enables effective multiplex detection by avoiding photo-bleaching and peak overlapping, allowing for precise identification of analytes using nanoaggregate-embedded beads with different probe molecules or Raman dyes, enhancing the accuracy of bio-detection processes.

Implementation Method 1

detecting Raman spectra of the product formed by binding of the sensor bead and an analyte in a sample

Methodology Applied

Scientific EffectRaman scattering:

Implementation Method 2

adding a Raman dye into a solution of metal nanoparticles to generate nanoaggregates

Methodology Applied

Scientific EffectSurface plasmon resonance:

Data Source

PatentUS8187820B2Detection method using nanoaggregate-embedded beads and system thereof

Publication Date: 2012.05.29 NATIONAL CHUNG CHENG UNIV

AI summary

The invention discloses a detection method using nanoaggregate-embedded beads and system thereof, which are characterized in that the nanoaggregate of Raman dye and metal nanoparticles is coated by an inorganic oxide to form a nanoaggregate-embedded bead, and which is then conjugated with a probe molecule to form a sensor bead. The Raman spectra of the product formed by binding of the sensor bead and an analyte in a sample is detected for determining whether the analyte exists in the sample. In embodiment, the pH of the solution of metal nanoparticles is controlled to keep at 10, and the concentration of the Raman dye is controlled to keep between 1×10−6 M and 2×10−6 M for reducing the size of the nanoaggregate.