Nanofraction Immune Modulators for T Cell Regulation
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Solution Overview
Problem
Current understanding of immune modulation is limited, particularly regarding small molecules that influence cell-mediated immunity, and there is a need for effective methods to enhance or suppress immune responses in a controlled manner.
Innovation Solution
Development of nanofraction molecules, obtained from sources like colostrum and eggs, which are purified and administered to modulate cell-mediated immunity by enhancing or suppressing immune responses, using techniques such as phase separation, filtration, and dialysis to create specific molecular weight fractions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If nanofraction molecules are purified from colostrum or eggs using phase separation, filtration, and dialysis, then immune modulation effectiveness is improved, but manufacturing complexity increases
Solution Approach 1:
The patent divides the complex mixture of colostrum or egg components into distinct molecular weight fractions using sequential filtration steps (10,000 Da, then 3,000 Da membranes) and dialysis, isolating the active nanofraction molecules from other biological components. This segmentation enables targeted immune modulation while simplifying the active ingredient profile.
Solution Approach 2:
The patent extracts the active nanofraction molecules (3,000-10,000 Da) from the complex biological matrices of colostrum or eggs through a multi-step purification process involving phase separation, ultrafiltration, and dialysis, separating the immunologically active components from proteins, fats, and other macromolecules.
2Productivity
If source animals are exposed to greater amounts of antigens to enhance nanofraction production, then immune modulator yield is improved, but animal welfare and safety concerns worsen
Solution Approach 1:
The patent employs preliminary immunization of source animals with specific antigens before harvesting colostrum or eggs, allowing the animals' immune systems to naturally produce the desired nanofraction molecules during normal physiological processes, avoiding the need for excessive antigen exposure during production.
Solution Approach 2:
The patent utilizes the source animals' own immune systems to naturally produce nanofraction molecules through controlled antigen exposure and normal physiological processes, allowing the animals to self-regulate their immune response and produce the desired biomolecules without external intervention or stress.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The nanofraction molecules effectively modulate immune responses, as demonstrated by increased or decreased ATP production in immune cells, indicating their ability to enhance or down-regulate immune activity, thereby improving immune function and balancing immune responses.
Implementation Method 1
Known processes may be used to partially, substantially, or completely purify nanofraction immune modulators from other molecules present in the nanofraction source animal from which they are obtained and, optionally, to concentrate the nanofraction immune modulators. Such processes include, without limitation, mechanical separation, phase separation
Implementation Method 2
Such processes include, without limitation, mechanical separation, phase separation (e.g., separation of aqueous and non-aqueous components from one another), precipitation, centrifugation, filtration (including micro filtration, with a molecule weight cutoff (MWCO) in the range of about 12,000 Da down to about 4,000 Da, and nanofiltration, with an MWCO of less than about 4,000 Da)
Implementation Method 3
Such processes include, without limitation, mechanical separation, phase separation (e.g., separation of aqueous and non-aqueous components from one another), precipitation, centrifugation, filtration (including micro filtration, with a molecule weight cutoff (MWCO) in the range of about 12,000 Da down to about 4,000 Da, and nanofiltration, with an MWCO of less than about 4,000 Da), dialysis
Data Source
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AI summary
Compositions that include extracts from sources of immune modulators that include nanofraction immune modulator molecules (i.e., molecules having molecular weights of about 3,000 Da and less) are disclosed. These compositions may also include other immune modulators, such as transfer factor. Administration of compositions with extracts that include nanofraction immune modulator molecules modulates the cell mediated immunity (e.g., down regulates undesired T cell activity) of a subject to which such compositions are administered. When administered with transfer factor, the combination of nanofraction immune modulator molecules and transfer factor down regulates undesired T cell activity while increasing, or up regulating, T cell activity against pathogens and other undesirable entities, such as cancer cells and other aberrant or mutated cells. Assays and assay techniques for evaluating the immune modulation capabilities of various substances are also disclosed.