NADH and Ethanol Biosensing Chip Using Nanogold Nickel Hexacyanoferrate
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Solution Overview
Problem
Current ethanol detection methods in fermentation processes are slow, require pre-treatment, and cannot provide real-time concentration values, lacking effective technologies for online monitoring of ethanol and NADH.
Innovation Solution
A biosensor is developed using a nanocomposite material of gold nanoparticles and nickel hexacyanoferrate, combined with ethanol dehydrogenase, which is prepared through a simple and cost-effective process, enabling real-time detection of ethanol and NADH through electrochemical methods.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional ethanol detection methods (spectrophotometry, chromatography, colorimetry) are used, then detection accuracy can be maintained, but detection time is prolonged and real-time monitoring is unable to be achieved
Solution Approach 1:
The patent replaces conventional mechanical/chemical detection methods (spectrophotometry, chromatography, colorimetry) with an electrochemical biosensing system. The biosensor uses ethanol dehydrogenase to catalyze ethanol oxidation, generating electrical signals that can be measured in real-time, thus substituting time-consuming laboratory methods with rapid electrochemical detection while maintaining accuracy
Solution Approach 2:
The patent introduces an intermediary substance - the biosensing chip containing ethanol dehydrogenase and nickel hexacyanoferrate - that mediates between ethanol and the detection system. This intermediary enables real-time conversion of chemical information into electrical signals, bridging the gap between accurate chemical detection and rapid measurement
2Measurement precision
If conventional detection methods are used, then comprehensive analysis can be performed, but pre-treatment requirements increase process complexity
Solution Approach 1:
The biosensing chip performs self-service by containing all necessary components (enzyme, mediator, conductive material) integrated in a single device. The ethanol dehydrogenase automatically catalyzes ethanol oxidation when ethanol contacts the sensor surface, eliminating the need for external pre-treatment steps such as sample preparation, reagent addition, or instrument calibration that characterize conventional methods
3Productivity
If online real-time monitoring technology is developed, then detection speed is improved, but technological complexity and development difficulty increase
Solution Approach 1:
The patent achieves real-time monitoring by changing key parameters of the detection system: using nanoscale nickel hexacyanoferrate particles to enhance electrochemical activity, optimizing enzyme immobilization conditions, and adjusting screen printing parameters. These parameter optimizations enable rapid detection without requiring complex system architecture
Solution Approach 2:
The patent applies local quality by concentrating functional materials (nickel hexacyanoferrate nanoparticles, ethanol dehydrogenase) specifically at the sensing interface of the working electrode. This localized functionalization creates a highly active detection zone that achieves real-time monitoring capability without requiring the entire system to be complex
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The biosensor achieves rapid, dilution-free detection of ethanol with a wide linear range, providing real-time monitoring of fermentation broth and maintaining high sensitivity and stability over time.
Implementation Method 1
The core of electrochemical sensors lies in the sensing electrodes, including the development of high-performance sensing materials
Implementation Method 2
the concentration of ethanol is one of the main parameters of fermentation, which on the one hand influences the growth of yeast and on the other hand influences the catalytic performance of various enzymes involved in the fermentation process
Implementation Method 3
The gold nanoparticles/nickel hexacyanoferrate/carbon mixed ink is fixed on a support by the screen-printing technique to form a working electrode
Implementation Method 4
An ethanol dehydrogenase mixed solution containing a certain amount of glutaraldehyde is prepared. A certain amount of the mixed enzyme solution is taken and evenly applied on the working electrode
Data Source
AI summary
The invention relates to a simple method for preparing an NADH and ethanol biosensing chip, applicable to NADH or ethanol detection in the fermentation field, clinical medicine and food engineering. The sensing material described in the present invention is simple in preparation and can be prepared in batches, the nanogold is uniformly distributed on the surface of nickel hexacyanoferrate, and the quality of the sensing chip prepared based on this material is controllable. The sensing chip uses ethanol dehydrogenase as a biorecognition element and is more selective. The sensor chip detects ethanol and NADH in a wide linear range without dilution at a single detection time of less than 30 s and can realize real-time monitoring of fermentation broth.