Nanopore Analyte Detection System for Concentration and Binding Analysis
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Solution Overview
Problem
Current methods for detecting biologically active components in fluids are often expensive, time-consuming, and limited in their ability to analyze multiple components simultaneously, leading to diagnostic delays and reduced accuracy.
Innovation Solution
A nanopore-based system that includes an analyte detection complex with an analyte ligand, threading element, and signal elements, which is used to determine analyte concentration and assess binding interactions by applying voltages across a nanopore assembly to detect binding and dissociation signals.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional detection methods are used, then detection capability is provided, but cost and time consumption increase significantly
Solution Approach 1:
The detection system is segmented into multiple independent nanopore assemblies, each capable of detecting different analytes simultaneously. This parallel segmentation allows multiple detection operations to occur at the same time, dramatically reducing total test time while maintaining individual detection precision through each nanopore assembly's specialized ligand configuration.
Solution Approach 2:
The nanopore assembly platform is designed with universal functionality to detect multiple types of analytes (proteins, small molecules, nucleic acids) through configurable ligands. This multi-functionality eliminates the need for separate dedicated detection systems for each analyte type, reducing overall cost and time by consolidating multiple detection capabilities into a single platform.
2Measurement precision
If traditional detection methods are used, then detection capability is provided, but multiple components cannot be analyzed simultaneously
Solution Approach 1:
The system divides the detection capacity into multiple independent nanopore assemblies, each configured with specific ligands for different analytes. This segmentation enables simultaneous analysis of multiple components in parallel, with each nanopore assembly dedicated to a specific target, thereby achieving both high detection precision and multi-component versatility.
Solution Approach 2:
The system achieves versatility by changing the ligand parameters on nanopore assemblies to match different analyte specificities. By configuring different ligands (proteins, antibodies, aptamers) on different nanopore assemblies, the same platform can adapt to detect various analyte types simultaneously, enhancing both detection capability and multi-component analysis ability.
3Measurement precision
If traditional detection methods are used, then detection capability is provided, but concentration determination is not available
Solution Approach 1:
The system incorporates feedback mechanisms where the binding events detected at nanopore assemblies are quantified to determine analyte concentration. By measuring the number of binding events and comparing against control values, the system provides feedback that enables concentration determination, transforming simple detection into quantitative measurement capability.
Solution Approach 2:
The system uses control nanopore assemblies with known concentrations to create reference copies for comparison. By comparing the binding counts from test samples against these control references, the system can determine analyte concentration through relative measurement, adding quantitative capability while maintaining the core detection function.
4Measurement precision
If traditional detection methods are used, then detection capability is provided, but accuracy is reduced due to complexity
Solution Approach 1:
The system extracts the complex detection function into simplified nanopore assemblies, each handling a specific analyte type independently. By taking out the complexity from a single monolithic system and distributing it across multiple simple, specialized units, the overall system achieves high accuracy through simplicity at each individual nanopore level while maintaining multi-analyte capability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid, cost-effective detection and analysis of multiple biologically active components, improving diagnostic efficiency and accuracy by determining concentration and binding strengths.
Implementation Method 1
nanopore-based methods, compositions, and systems for assessing analyte-ligand interactions and analyte concentration in a fluid solution
Implementation Method 2
As a first voltage is applied across the nanopore assembly, the analyte ligand is presented to an analyte in the solution. As a second voltage that is opposite in polarity to the first voltage is applied across the nanopore assembly, the analyte binds to the analyte ligand
Data Source
AI summary
Provided are nanopore-based methods, compositions, and systems for assessing analyte-ligand interactions and analyte concentration in a fluid solution. The compositions include an analyte detection complex that is associated with a nanopore to form a nanopore assembly, the analyte detection complex including an analyte ligand. As a first voltage is applied across the nanopore assembly, the analyte ligand is presented to an analyte in the solution. As a second voltage that is opposite in polarity to the first voltage is applied across the nanopore assembly, the analyte binds to the analyte. By comparing the total number of analyte-ligand binding pairs to a control binding count, the concentration of the analyte can be determined. In other examples, further increasing the second voltage can result in dissociation of the analyte-ligand pair, from which a dissociation voltage—and hence a dissociation constant—can be determined.