Nanopore Genetic Marker Analysis via Probe Hybridization

The patent replaces the mechanical gel electrophoresis system with a nanopore-based detection system. The nanopore device directly detects DNA sequences through electrical current measurements as molecules pass through the pore, eliminating the need for gel matrices, electrophoresis chambers, and manual staining procedures. This substitution of mechanical/chemical separation methods with direct electrical detection achieves faster analysis while maintaining simplicity through automated signal processing

The patent uses probe molecules that bind to complementary sequences on the DNA target. These probes create hybridized structures that can be detected through the nanopore, effectively copying the detection function. The probes act as intermediaries that transform the DNA sequence information into detectable signal patterns, enabling rapid identification without direct manipulation of the target DNA through complex mechanical processes

The nanopore system performs automated detection and analysis without requiring manual intervention for each measurement step. The system automatically captures current traces, processes signals, and identifies genetic markers based on electrophoretic mobility and sequence-specific probe binding. This self-service capability eliminates repetitive manual operations while maintaining ease of use through standardized protocols and automated data interpretation

The patent employs pre-designed probe molecules that are prepared in advance with specific sequences complementary to target markers. These probes are pre-attached or pre-positioned to bind to their targets before analysis, eliminating the need for manual probe synthesis or complex pre-processing steps during the actual measurement. This preliminary preparation simplifies operation while enabling high-throughput analysis