Nanopore Analysis Device Third Electrode Transfer Frequency
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Solution Overview
Problem
Conventional nanopore-based analysis methods face challenges in improving the transfer frequency of biological polymers through nanopores, particularly for low concentration samples, leading to reduced throughput in measurements.
Innovation Solution
The equipment includes a chamber with electrodes and a nanopore, where an additional electrode is placed near the nanopore to enhance the electric potential difference, allowing for efficient collection and transfer of biological polymers by applying specific voltage configurations between the electrodes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional nanopore-based analysis methods are used, then the measurement can be performed with simple structure, but the transfer frequency of biological polymers through nanopore is low leading to reduced throughput
Solution Approach 1:
The electrode system is segmented into multiple electrodes (first electrode in sample introduction section, second electrode in sample outflow section, and third electrode near nanopore). This segmentation allows independent control of electric potential in different regions, enabling enhanced transfer frequency through the third electrode while maintaining overall system functionality through the first and second electrodes.
Solution Approach 2:
The third electrode is specifically positioned near the nanopore to create a localized electric potential difference that enhances the transfer frequency of biological polymers through the nanopore. This local enhancement does not require increasing the potential difference across the entire chamber, thus improving productivity without proportionally increasing overall device complexity.
2Productivity
If voltage is applied to increase electric potential difference for collecting biological polymers, then transfer frequency improves, but energy consumption increases
Solution Approach 1:
The third electrode creates a localized electric potential difference specifically near the nanopore where it is most needed for enhancing transfer frequency. This localized approach concentrates energy application where it provides maximum benefit, rather than applying high voltage across the entire sample introduction section, thus improving productivity while moderating overall energy consumption.
Solution Approach 2:
The voltage applying member can dynamically adjust the potential difference between the third electrode and other electrodes based on measurement conditions. This dynamic control allows optimization of transfer frequency while managing energy consumption, particularly for low concentration samples where enhanced collection is most beneficial.
3Productivity
If additional electrode is added near nanopore to enhance electric potential difference, then measurement throughput improves, but device complexity increases
Solution Approach 1:
The additional third electrode segments the electric field control into distinct regions, allowing independent optimization of the nanopore region for enhanced throughput. While this adds a device element, the segmentation enables functional improvements that justify the added complexity by significantly increasing measurement throughput.
Solution Approach 2:
The third electrode serves multiple functions: it enhances the electric potential difference near the nanopore for improved transfer frequency, assists in collecting biological polymers, and works in coordination with the first and second electrodes for overall system control. This multi-functionality maximizes the benefit of the added electrode while managing device complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly increases the transfer frequency of biological polymers through the nanopore, thereby enhancing the measurement throughput and efficiency, especially for low concentration samples.
Implementation Method 1
applying voltage to an electrode provided near the nanopore to increase an electric potential difference near the nanopore
Implementation Method 2
transferring a charged polymer molecule according to an electric field gradient
Data Source
AI summary
The biological polymer analyzing equipment with nanopore includes a chamber part having a chamber having a sample introduction section and a sample outflow section separated by a substrate; a first electrode provided in the sample introduction section and a second electrode provided in the sample outflow section; a thin membrane formed on the substrate; a nanopore provided in the thin membrane of the substrate and communicating between the sample introduction section and the sample outflow section; a third electrode provided near the nanopore of the substrate; and a voltage applying member to electrodes, wherein the voltage applying member includes a member for applying voltages between the first electrode and the third electrode, between the first electrode and the second electrode, respectively, and between the third electrode and the second electrode, and relates to a method for analyzing a biological polymer using the biological polymer analyzing equipment with nanopore.