Nanoporous Microsponge Particles via Organic Cross-Linking
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Solution Overview
Problem
Conventional methods for producing nanoporous microparticles for biomolecule delivery often involve toxic elements and harsh conditions, limiting their safety and efficiency as carriers for drugs and biomolecules.
Innovation Solution
A method involving cross-linking/precipitation of hyaluronic acid with a cross-linking agent prepared from 1,1'-carbonyldiimidazole and a diamine compound, producing nanoporous microsponge particles with a high swelling ratio suitable for drug delivery, characterized by an average molecular weight range of 10KDa to 200KDa, and specific dimensions and pore sizes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Shape
If inorganic components (metal salts) are added to produce nanoporous microparticles, then porous structure and high surface-to-volume ratio are achieved, but toxic elements and harsh conditions are introduced
Solution Approach 1:
The invention extracts and removes the harmful inorganic metal salt components from the nanoparticle synthesis process. Instead of using metal phosphates (calcium, manganese, copper) as structure-forming agents, the patent employs purely organic crosslinking agents that form hydrogen-bonded networks, eliminating toxic elements while preserving the desired porous microsponge architecture.
Solution Approach 2:
The invention introduces organic crosslinking agents as intermediary substances that mediate the formation of nanoporous structures without introducing toxicity. These organic mediators (such as glutaraldehyde, genipin, or EDC/NHS couples) facilitate the crosslinking of polysaccharide chains to create the porous network, replacing the role of inorganic metal salts while being biocompatible and non-toxic.
2Stability of the object's composition
If high molecular weight polysaccharides are used, then structural stability is improved, but swelling ratio decreases
Solution Approach 1:
The invention optimizes the molecular weight parameter of the polysaccharide precursors to a specific range (10-200 KDa) that balances structural stability and swelling capacity. This parameter optimization ensures that the chains are long enough to form stable crosslinked networks but short enough to allow adequate water penetration and swelling, achieving both structural integrity and high drug loading capacity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method enables the production of biocompatible nanoporous microsponge particles with high loading and sustained release capabilities for proteins and other biomolecules, enhancing their bioavailability and suitability for regenerative medicine and pharmaceutical applications without using toxic metal ions.
Implementation Method 1
a cross-linking/precipitation step wherein a cross-linking agent reacts with hyaluronic acid having nucleophile functional groups which react with said cross-linking agent
Implementation Method 2
The swelling capability of nanoporous microsponge particles is a telling ex-post measurement of capillary suction of a nanoporous microsponge particle
Implementation Method 3
said a cross-linking agent being prepared by a reaction between 1,1'-carbonyldiimidazole (CDI) and a diamine compound
Implementation Method 4
a cross-linking/precipitation step wherein a cross-linking agent reacts with hyaluronic acid
Data Source
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Figure 2A~2D
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AI summary
A method to produce nanoporous microsponge particles useful as carriers for drug delivery. The method comprises a cross-linking/precipitation step wherein a cross-linking agent reacts with a biocompatible polymer having nucleophile functional groups, which react with said cross-linking agent. The cross-linking agent is prepared by a reaction between 1,1'-carbonyldiimidazole (CDI) and a diamine compound. The biocompatible polymer has an average molecular weight ranging from 10KDa to 150KDa.