Nanostructure EV Capture for Direct miRNA Extraction
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Solution Overview
Problem
Existing methods for collecting extracellular vesicles (EVs) from sample solutions, such as ultracentrifugation and aggregation reagent methods, are cumbersome and result in loss of EVs during the process, necessitating a more efficient and simple device for capturing and preserving EVs.
Innovation Solution
A device comprising a nanostructure body, such as nanowires or cellulose fibers, capable of capturing EVs directly from a sample solution, allowing for direct extraction of miRNA without separating the EVs, and enhancing preservation stability and transfer convenience.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If ultracentrifugation is used to collect EVs from sample solution, then EVs can be separated, but the work procedure increases and EV loss occurs during fraction collection
Solution Approach 1:
The patent extracts the EV capture function from the complex ultracentrifugation process by introducing a specific capture device that can directly bind EVs from sample solution, eliminating the need for fraction collection and reducing procedural complexity
Solution Approach 2:
The patent uses a capture device as an intermediary component that facilitates EV isolation by providing a specific binding surface, thereby simplifying the overall separation process and reducing EV loss during handling
2Quantity of substance
If ultracentrifugation is used to collect EVs from small volume samples, then EVs can be concentrated, but EV loss increases during the operation process
Solution Approach 1:
The patent extracts EVs directly from small volume samples using a capture device that minimizes handling steps, thereby concentrating EVs while reducing loss that typically occurs during fraction collection in ultracentrifugation
Solution Approach 2:
The capture device enables EV concentration through direct binding from the sample solution, allowing the system to self-concentrate EVs without requiring additional manipulation steps that would increase loss
3Reliability
If aggregation reagent method is used to separate EVs, then EVs can be aggregated, but the work procedure increases and EV loss occurs during separation
Solution Approach 1:
The patent removes the need for aggregation reagents and subsequent separation steps by using a capture device that directly binds EVs from the sample solution, thereby simplifying the workflow and reducing EV loss
Solution Approach 2:
The capture device serves as an intermediary that replaces the aggregation reagent function, providing direct EV binding capability and eliminating the need for additional separation operations
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device enables efficient capture and preservation of EVs with reduced loss, facilitating direct miRNA extraction and convenient transfer, particularly suitable for small sample volumes.
Implementation Method 1
a nanostructure body capable of capturing extracellular vesicles
Data Source
AI summary
The present invention provides a novel miRNA extraction method and a method for analyzing miRNA extracted by using said miRNA extraction method. According to the present invention, provided is, for example, a method for extracting miRNA from extracellular vesicles in a sample solution, by using a device capable of capturing extracellular vesicles, the miRNA extraction method comprising: an extracellular vesicle capturing step for capturing extracellular vesicles in a sample solution onto a device by bringing the sample solution and the device in contact with each other; and a miRNA extraction step for homogenizing the extracellular vesicles by bringing the device having captured the extracellular vesicles in contact with a homogenization liquid for extracellular vesicles to extract miRNA from the extracellular vesicle into the homogenization liquid.