Biosensor Reagent Composition Using Naphthol Green B
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Solution Overview
Problem
Existing biosensors face challenges in achieving high sensitivity and minimizing blood sample volume for glucose measurement, particularly in miniaturized formats, which affects user convenience and accuracy.
Innovation Solution
A reagent composition for biosensors incorporating Naphthol Green B and a ruthenium complex, along with an enzyme, is used to enhance electron transfer efficiency, including surfactants and water-soluble polymers to stabilize the enzyme and improve reaction efficiency on the electrode surface.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If the biosensor is miniaturized to reduce blood sample volume, then the amount of blood needed for measurement is reduced, but the detection sensitivity and analysis linearity deteriorate
Solution Approach 1:
The patent uses a composite reagent system combining Naphthol Green B (a redox mediator) with ruthenium complexes and enzymes. This composite material approach enhances electron transfer efficiency and catalytic activity, enabling high sensitivity detection even in miniaturized biosensors with limited sample volumes. The synergistic interaction between multiple components compensates for the reduced reaction volume.
Solution Approach 2:
The patent optimizes the concentration ratios and physical-chemical parameters of the reagent components. By adjusting the concentration of Naphthol Green B relative to the enzyme and ruthenium complex, the system achieves maximum electron transfer efficiency. This parameter optimization enables the biosensor to maintain high detection sensitivity with minimal blood sample volume (0.3-2 μL).
2Measurement precision
If the concentration of electron transfer mediator is increased to improve electron transfer efficiency, then the detection sensitivity improves, but the cost and complexity of the reagent composition increases
Solution Approach 1:
The patent employs Naphthol Green B as an intermediary redox mediator that facilitates electron transfer between the enzyme's active site and the electrode. This mediator acts as a bridge, enabling efficient electron transfer at low concentrations. The intermediary approach avoids the need for high concentrations of direct electron acceptors, simplifying the reagent composition while maintaining high detection sensitivity.
Solution Approach 2:
The patent replaces complex multi-component enzyme systems with a streamlined reagent composition centered on Naphthol Green B as the primary electron transfer mediator. This substitution simplifies the reagent formulation while achieving comparable or superior electron transfer efficiency through the unique redox properties of Naphthol Green B in combination with ruthenium complexes.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composition significantly increases glucose detection performance, improves analysis linearity, and reduces the amount of blood needed for measurement, making it suitable for frequent and accurate glucose monitoring.
Implementation Method 1
the reduced FADH 2 is oxidized to FAD through an oxidation-reduction reaction with the electron transfer mediator, and the electron transfer mediator is reduced. Electrons generated from the electron transfer mediator in a reduction state formed in this manner disperse to an electrode surface
Implementation Method 2
the blood glucose is oxidized to gluconate due to a catalytic action of a glucose oxidase. At this time, FAD, which is an active site of the glucose oxidase, is reduced and became FADH 2
Data Source
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AI summary
This invention discloses a reagent composition for a biosensor having high sensitivity which is capable of improving analysis linearity of an analyte such as glucose by reacting an oxidoreductase, a metal-containing complex, and Naphthol Green B, and minimizing blood necessary for measuring blood glucose since it is possible to detect a concentration of a small amount of the analyte, and a biosensor having the same. The reagent composition for a biosensor includes at least two kinds of electron transfer mediators including Naphthol Green B, and an enzyme.