Aqueous Buffered Naphthyl Compounds for Universal Mycotoxin Extraction
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Solution Overview
Problem
Current methods for extracting mycotoxins from food and animal feed are inefficient and environmentally harmful due to the use of large quantities of organic solvents, and lack a universal extraction agent for the diverse range of mycotoxins with varying solubility properties.
Innovation Solution
The use of aqueous, buffered naphthyl and/or phenyl compounds or their heterocyclic analogues with sulphonic acid or carbonate acid groups to uniformly extract hydrophobic and hydrophilic mycotoxins from food and animal feed, allowing for a single extraction agent to handle a wide range of mycotoxin groups.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If organic solvents (ethanol, acetonitrile, methanol) are used for extraction, then extraction efficiency of mycotoxins is improved, but large quantities of organic solvents are created that must be disposed of
Solution Approach 1:
The invention changes the chemical parameters of the extraction medium by using aqueous buffered solutions with specific pH ranges (pH 2.0-12.0, preferably pH 3.0-10.0) instead of traditional organic solvents. This parameter change allows effective extraction of mycotoxins while eliminating the need for large quantities of organic solvents and their associated disposal problems.
2Measurement precision
If different extraction methods are used for different mycotoxins, then extraction accuracy is improved, but device complexity and process time increase
Solution Approach 1:
The invention creates a universal extraction method using aqueous buffered solutions that can extract multiple types of mycotoxins (aflatoxins, ochratoxin, deoxynivalenol, fumonisins, zearalenone, T2/HT2 toxins) simultaneously. This single multi-functional approach replaces multiple separate extraction procedures, reducing process complexity while maintaining extraction accuracy for all mycotoxin types.
3Reliability
If multiple solvents are used for different mycotoxin groups, then extraction completeness is improved, but loss of time and resources increase
Solution Approach 1:
The invention merges the extraction of multiple mycotoxin groups into a single unified process using aqueous buffered solutions. Instead of performing separate extractions for hydrophobic aflatoxins and hydrophilic mycotoxins, the method combines all extractions into one step, reducing time and resource consumption while ensuring complete extraction of all mycotoxin types.
4Measurement precision
If special buffers are used for ochratoxin extraction, then extraction accuracy is improved, but ease of operation decreases
Solution Approach 1:
The invention makes the extraction method universal by using aqueous buffered solutions with pH 2.0-12.0 that work for all mycotoxins including ochratoxin, eliminating the need for special buffers. This simplifies the operation while maintaining extraction accuracy, as operators only need to prepare a single type of extraction solution regardless of which mycotoxins are being analyzed.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables efficient and environmentally friendly extraction of a broad spectrum of mycotoxins, reducing the need for multiple solvents and sample preparations, thereby saving time and resources while ensuring accurate quantification.
Implementation Method 1
aqueous, buffered naphthyl and/or phenyl compounds or their heterocyclic analogues with sulphonic acid or carbonate acid groups to uniformly extract hydrophobic and hydrophilic mycotoxins
Data Source
AI summary
The invention relates to a method for extracting mycotoxins from grain and other food products or from feed and its subsequent quantification. Fields of application are the food industry, the animal feed industry or biotechnology. The objective of the present invention is to develop an extraction method with which it is possible to uniformly extract mycotoxins with different dissolving properties. It was found that with the aid of aqueous, buffered naphthyl and/or phenyl compounds or their heterocyclical analogues, both hydrophobic and hydrophilic mycotoxins can be extracted. The method according to the invention is characterized in that the buffered solutions of naphthyl and/or phenyl compounds and/or their heterocyclical analogues, which carry at least one sulphonic acid or at least one carbonate acid group, are brought into contact with the grain or other food products or animal feed, the aqueous solution is then separated and the content of the extracted mycotoxins in the aqueous solution is determined.