Negative-Pressure Fluidic Degassing for Bubble-Free Sequencing
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Solution Overview
Problem
Gas present in sequencing reagents and nucleic acid samples forms bubbles in the flow cell, affecting image quality and sequencing accuracy in high-throughput gene sequencing.
Innovation Solution
A fluidic system with a degassing device connected to a pipeline to remove gas from liquid, using a sealed chamber under negative pressure to degas the liquid before it enters the reaction device, and a method to monitor the degassing device's functionality through air pressure changes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If gas is present in the sequencing reagents and nucleic acid sample, then the liquid can be delivered to the reaction device, but bubbles will form in the flow cell affecting image quality and sequencing accuracy
Solution Approach 1:
The patent applies preliminary action by implementing a degassing device that removes gas from liquid before the liquid enters the flow cell. The degassing device is positioned in the liquid delivery path upstream of the reaction device, ensuring that gas is removed in advance, preventing bubble formation during sequencing operations and maintaining image quality and sequencing accuracy.
2Reliability
If a degassing device is added to remove gas from liquid, then bubble formation is reduced, but the device complexity increases
Solution Approach 1:
The patent applies pneumatics and hydraulics by using a degassing device that utilizes pressure differential (negative pressure) to remove gas from liquid. The degassing device creates a pressure difference between the liquid inlet and outlet, causing gas bubbles to form and rise to the surface where they can be removed, thereby reducing bubble formation in the flow cell without requiring complex mechanical structures.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Reduces bubble formation in the reaction device, improving sequencing quality by ensuring gas-free liquid is used, and the method ensures the degassing device operates correctly, enhancing the reliability and efficiency of the fluidic system.
Implementation Method 1
the portion of the pipeline located within the sealed chamber is capable of, when the sealed chamber is under a negative pressure condition, allowing the gas in the liquid in the pipeline to permeate out
Implementation Method 2
allowing the gas in the liquid in the pipeline to permeate out, so that the liquid in the pipeline is degassed
Data Source
AI summary
The present application discloses a fluidic system, a control method, a sequencing system, and a storage medium. The fluidic system includes a reaction device, a pipeline, and a degassing device. The reaction device is configured to provide a site for carrying out biochemical reactions. The pipeline is in communication with the reaction device and configured to deliver liquid to the reaction device and/or receive liquid flowing out from the reaction device. The degassing device is connected to the pipeline and configured to remove gas from the liquid in the pipeline. In this way, by removing gas from the liquid in the pipeline using the degassing device, the formation of bubbles in the reaction device can be reduced.