Nervous System Extracellular Vesicle Capture Using APLP1 Antibodies
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing methods for collecting extracellular vesicles from nervous system cells are inefficient due to the lack of a protein specific to these cells, such as NCAM or CD171.
Innovation Solution
The use of immunoprecipitation targeting APLP1, a protein specifically expressed in nervous system cells, to form a complex with extracellular vesicles, followed by collection of this complex.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If non-specific proteins like NCAM or CD171 are used for collecting extracellular vesicles, then the collection process can be performed, but the collection efficiency is poor due to lack of nervous system cell specificity
Solution Approach 1:
The patent applies local quality by selecting APLP1 as the target protein specifically for nervous system cell-derived extracellular vesicles. APLP1 is a nervous system-specific protein that is not expressed in other cell types, providing localized specificity to the collection method. This resolves the contradiction by making the collection process both efficient and highly specific to nervous system cells through the use of anti-APLP1 antibodies.
2Reliability
If immunoprecipitation with anti-APLP1 antibody is used, then specificity to nervous system cells is improved, but the collection process becomes more complex
Solution Approach 1:
The patent uses anti-APLP1 antibodies as intermediaries to bridge the gap between the extracellular vesicles and the collection system. The antibodies specifically bind to APLP1 on the surface of nervous system cell-derived vesicles, enabling selective capture. This intermediary approach maintains high specificity while using well-established immunoprecipitation technology, thus not significantly increasing overall process complexity.
3Productivity
If crude purification methods are used before immunoprecipitation, then the overall collection efficiency is improved, but the purity of the final product may be compromised
Solution Approach 1:
The patent employs a segmented, multi-step purification approach. First, crude purification is performed to remove large debris and concentrate vesicles. Then, immunoprecipitation with anti-APLP1 antibodies is applied as a second stage to specifically isolate nervous system cell-derived vesicles. This segmentation allows each step to optimize for its specific function, achieving both high overall efficiency and high final purity.
Solution Approach 2:
The patent applies preliminary action by performing crude purification before the specific immunoprecipitation step. This preliminary concentration and cleanup step reduces the complexity of the subsequent immunoprecipitation, improving overall efficiency while the specific antibody step ensures final purity. The preliminary action prepares the sample in a way that enhances the effectiveness of the following specific isolation step.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method allows for the efficient collection of extracellular vesicles from nervous system cells, enabling accurate detection of neuropsychiatric disorders through biomarkers like tau protein and other biomolecules.
Implementation Method 1
mixing an anti-APLP1 antibody and a sample containing extracellular vesicles to form a complex of the anti-APLP1 antibody and the extracellular vesicle
Implementation Method 2
the crude purification is performed by a size exclusion chromatography
Implementation Method 3
the crude purification is performed by an ultracentrifugation
Implementation Method 4
the crude purification is performed by an affinity purification
Data Source
AI summary
The present invention addresses a problem of providing a method for collecting extracellular vesicles derived from nervous system cells at an improved efficiency.This problem is solved by a method for collecting extracellular vesicles derived from nervous system cells, said method comprising a step for mixing an anti-APLP1 antibody with a sample containing extracellular vesicles to form anti-APLP1 antibody-extracellular vesicle complexes and a step for collecting the anti-APLP1 antibody-extracellular vesicle complexes.