Neural Precursor Cell Populations for Nervous System Repair
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current treatments for central and peripheral nervous system disorders primarily manage symptoms rather than addressing the root cause, lacking effective methods for repairing or replacing damaged neural tissue.
Innovation Solution
Development of neural precursor cell populations that can migrate and differentiate into functional neurons, specifically enriched for markers such as AS1, ATRNL1, and CXCR4, allowing for transplantation to treat neural disorders by forming GABA-producing inhibitory interneurons.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If current symptom management therapies are used for nervous system disorders, then treatment simplicity is maintained, but the root cause of the disease cannot be addressed and neural tissue cannot be repaired
Solution Approach 1:
The patent uses neural precursor cells as intermediary agents that mediate between the administered therapy and the damaged neural tissue. These cells migrate to injury sites, differentiate into functional neurons, and restore neural circuitry, thereby addressing the root cause of neurological disorders while maintaining a relatively simple therapeutic administration process
2Reliability
If neural precursor cell transplantation is performed to repair damaged neural tissue, then the ability to address root cause and repair tissue is achieved, but the complexity of the treatment increases
Solution Approach 1:
The patent extracts and isolates specific neural precursor cells with defined migratory and differentiative capabilities from complex tissue sources. By purifying these cells based on specific markers and characteristics, the therapy achieves high efficacy while standardizing the product to reduce treatment complexity and improve manufacturing consistency
3Manufacturing precision
If enriched neural precursor cell populations are used for transplantation, then differentiation into functional inhibitory interneurons is improved, but the difficulty of cell isolation and purification increases
Solution Approach 1:
The patent employs fluorescently labeled antibodies that bind to specific cell surface markers on neural precursor cells. These fluorescent labels enable detection and sorting of the desired cell population through flow cytometry and other optical detection methods, thereby achieving high purification precision while simplifying the detection process through standardized fluorescent tagging techniques
Data Source
AI summary
The present invention provides cell populations enriched for specific neural precursor markers and methods of using such cell populations for treatment of disorders associated with dysregulation of inhibitory neuronal function and/or imbalances in excitatory/inhibitory neuronal activity. In particular, the present invention provides cell populations for use as a cell-based therapeutic, and methods for purification and use of these neural precursor cells in transplantation to ameliorate neural disorders associated with aberrant neural function.