Neurogranin-Related Peptide Stabilization with Organic Solvent
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Solution Overview
Problem
The analysis of neurogranin-related peptides in biological samples, particularly blood, is hindered by large variations in results due to the small amount of the peptide present and its degradation over time.
Innovation Solution
A method involving the preparation of a sample solution by mixing a biological sample with an organic solvent having a relative polarity of 0.200 to 0.700 and a final concentration of 5.0 to 20.0% (v/v) to stabilize the neurogranin-related peptide, followed by a purification process using affinity chromatography and mass spectrometry for analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If neurogranin-related peptide is detected directly from blood sample, then the analysis can be performed with minimal sample preparation, but the analysis results show large variations due to peptide degradation and small amount present
Solution Approach 1:
The patent applies preliminary action by adding organic solvent to the blood sample immediately during sample preparation to prevent peptide degradation before analysis. This pre-protective measure ensures peptide stability throughout the analysis process, resolving the contradiction between simple operation and precise measurement.
Solution Approach 2:
The organic solvent acts as an intermediary substance that mediates between the blood sample and the analysis system. It protects the neurogranin-related peptide from degradation while maintaining detectability, thus improving measurement precision without significantly complicating the overall analysis procedure.
2Measurement precision
If organic solvent is added to stabilize neurogranin-related peptide, then peptide degradation is suppressed and analysis result variation is reduced, but the sample preparation process becomes more complex
Solution Approach 1:
The patent applies parameter changes by optimizing the organic solvent concentration within a specific range (5-20% v/v) to achieve effective peptide stabilization without excessive complexity in sample preparation. This controlled parameter adjustment resolves the contradiction between improved measurement precision and increased process complexity.
3Quantity of substance
If neurogranin-related peptide amount in blood is very small, then the peptide can be detected as a biomarker, but large variation occurs in analysis results even in the same blood sample
Solution Approach 1:
The patent applies preliminary anti-action by adding organic solvent to counteract the degradation tendency of the neurogranin-related peptide before analysis. This pre-protective measure prevents the harmful degradation effect, ensuring reliable analysis results even for the small quantities of peptide present in blood samples.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method effectively suppresses peptide degradation and variation in analysis results, ensuring consistent detection of neurogranin-related peptides over time.
Implementation Method 1
mixing a biological sample containing a neurogranin-related peptide with an organic solvent having a relative polarity of 0.200 or more and 0.700 or less to prepare a sample solution
Implementation Method 2
a purification process using affinity chromatography
Data Source
AI summary
Provided is a method for analyzing a neurogranin-related peptide capable of suppressing variations in analysis results, and a method for preparing a biological sample containing a neurogranin-related peptide used therein. The method includes mixing a biological sample containing a neurogranin-related peptide with an organic solvent having a relative polarity of 0.200 or more and 0.700 or less to prepare a sample solution having a final concentration of the organic solvent of 5.0 (v/v) % or more.