NGF-Fc Plasmid Expression Control for Homeostasis Balance

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Solution Overview

Problem

Bioactive molecules such as toll-like receptors, enzymes, and hormones, when over-expressed, under-expressed, or mis-expressed, lead to homeostasis disruption and subsequent diseases, necessitating therapies to regulate their production and function.

Innovation Solution

Compositions comprising recombinant plasmids encoding nucleotide sequences that upregulate the production of mRNA sequences for fusion proteins like TLR3-Fc, TLR9-Fc, DNAse I-Fc, NGF-Fc, or insulin-Fc, administered via vectors like adeno-associated viruses or lipid vesicles to increase endogenous production of these biomolecules.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If recombinant plasmids are administered to upregulate production of fusion proteins, then bioavailability of target biomolecules increases, but risk of over-expression and homeostasis disruption increases

Engineering Contradiction:

Improvebioavailability of target biomoleculeVSAvoidhomeostasis disruption

Core Design Contradiction:

Quantity of substanceVSObject-affected harmful factors

Solution Approach 1:

The plasmid composition includes regulatory elements that enable feedback control of gene expression, allowing the system to automatically adjust production levels of fusion proteins to maintain homeostasis and prevent over-expression-related harmful effects while ensuring sufficient bioavailability of target biomolecules

Inventive Principle:

Principle #23Feedback

Solution Approach 2:

The invention utilizes inducible promoters and regulatory sequences that allow dynamic control of gene expression parameters, enabling adjustment of fusion protein production levels in response to physiological conditions, thereby increasing bioavailability when needed while preventing homeostasis disruption through controlled expression

Inventive Principle:

Principle #35Parameter changes

2Reliability

If plasmid composition is used to increase endogenous production of biomolecules, then disease prevention and treatment efficacy improves, but complexity of gene regulation mechanisms increases

Engineering Contradiction:

Improvedisease prevention efficacyVSAvoidgene regulation mechanism

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The plasmid composition employs universal regulatory elements and promoters that can control expression of multiple different fusion proteins (TLR3-Fc, TLR9-Fc, DNAse I-Fc, NGF-Fc, insulin-Fc) through a single standardized regulatory framework, reducing the complexity burden while maintaining reliable disease prevention efficacy across different therapeutic applications

Inventive Principle:

Principle #6Universality (Multi-functionality)

Data Source

PatentUS12529075B2Plasmid encoding a NGF and Fc fusion protein

Publication Date: 2026.01.20 WYVERN PHARMACEUTICALS INC

AI summary

Some embodiments of the present disclosure relate to one or more compositions that upregulate the production of one or more sequences of mRNA. The sequences of mRNA may encode for translation of a target biomolecule, thereby causing an increase in bioavailability of the target biomolecule within a subject that is administered the one or more compositions. In some embodiments of the present disclosure, the target biomolecule is a fusion protein with an Fc fragment, such as a toll-like receptor 3-Fc (TLR3-Fc). In some embodiments of the present disclosure, the target biomolecule is toll-like receptor 9-Fc (TLR9-Fc). In some embodiments of the present disclosure, the target biomolecule is deoxyribonuclease I-Fc (DNAse I-Fc). In some embodiments of the present disclosure, the target biomolecule is neural growth factor-Fc (NGF-Fc). In some embodiments of the present disclosure, the target biomolecule is insulin-Fc.