Nile Blue Reagent for Platelet Measurement Accuracy
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Solution Overview
Problem
Existing methods for measuring platelets in blood samples are affected by contaminants like fragmented red cells and lipids, leading to inaccurate results, especially in samples with low platelet counts, and dye degradation over time reduces measurement accuracy.
Innovation Solution
A reagent kit using Nile Blue hydrogensulfate or Nile Blue with an acid as a dye, combined with a buffering agent, to enhance platelet staining and storage stability, allowing for clearer differentiation of platelets from contaminants and improved measurement accuracy through flow cytometry.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of stationary object
If conventional dyes are used for platelet staining, then platelet measurement can be performed, but the dye degrades over time reducing measurement accuracy
Solution Approach 1:
The patent changes the chemical parameters of the dye by selecting specific dyes (Nile Blue, Thiazole Orange, Thiazole Orange hydrogensulfate, or Thiazole Orange fluorosulfonate) with stable chemical structures that resist degradation. This parameter change in dye selection maintains both long-term stability and measurement precision simultaneously
2Reliability
If platelet measurement is performed in samples with low platelet counts, then clinical diagnostic value is improved, but contaminants affect measurement accuracy more significantly
Solution Approach 1:
The patent utilizes fluorescent color changes of specific dyes when bound to platelets. The dyes exhibit distinct fluorescence characteristics that allow differentiation of platelets from contaminants based on their optical properties, enabling accurate measurement even in low platelet count samples
Solution Approach 2:
The patent replaces conventional light scattering-based detection with fluorescence-based detection. This substitution provides higher signal-to-noise ratio and better discrimination capability, improving measurement precision in samples with low platelet counts where contaminants are more significant
3Productivity
If flow cytometry is used for rapid blood cell measurement, then measurement speed is improved, but contaminants like fragmented red cells and lipids interfere with platelet detection
Solution Approach 1:
The patent employs fluorescent dyes that produce distinct color/fluorescence signals when bound to platelets, enabling optical differentiation from contaminants. This allows rapid flow cytometry measurement while maintaining precision by identifying platelets through their unique fluorescent characteristics rather than size alone
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution provides higher accuracy in measuring platelets by effectively distinguishing them from contaminants and maintaining dye stability over time, even in samples with low platelet counts, ensuring reliable results.
Implementation Method 1
Nile Blue having hydrogensulfate ion as a counter ion... as a dye for staining platelets... applying light to cells in the obtained measurement sample to detect scattered light and fluorescence emitted from the cells
Data Source
AI summary
A reagent for measuring platelets comprising Nile Blue hydrogensulfate, or Nile Blue and an acid, a reagent kit for measuring platelets comprising the reagent for measuring platelets, and a method for measuring platelets using the reagent or reagent kit.