Near-Infrared Fluorescence Reference Plates for Stable Intensity Measurement
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Solution Overview
Problem
The existing methods for measuring relative fluorescence intensity of near-infrared fluorescence are unreliable due to variations in rhodamine-based fluorescent dye solutions, which have low heat resistance and fluctuating fluorescence intensity over time, making it difficult to accurately measure near-infrared fluorescence intensity.
Innovation Solution
A method using plate-shaped or film-shaped molded bodies of resin compositions containing near-infrared fluorescent dyes, with identical and blank plates, to measure relative fluorescence intensity by calculating the difference in fluorescence intensities at specific wavelengths, ensuring uniform dispersion and stability of the dye.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If rhodamine-based fluorescent dye solution is used as a standard sample, then measurement can be performed, but the fluorescence intensity varies over time and between preparations, reducing measurement reliability
Solution Approach 1:
The patent changes the physical state parameter of the standard sample from liquid solution to solid molded body. This parameter change eliminates the temporal degradation and preparation variations inherent in liquid solutions, providing a stable, reproducible standard sample that can be stored and used repeatedly without degradation of fluorescence intensity.
Solution Approach 2:
The patent creates a composite material by incorporating the near-infrared fluorescent dye into a resin matrix to form a molded body. This composite structure provides both the fluorescent properties needed for measurement and the structural stability of the resin, eliminating the instability of pure dye solutions while maintaining measurement capability.
2Manufacturing precision
If melt-kneading is used to disperse fluorescent dye in resin, then uniform dispersion can be achieved, but the dye may decompose at high temperatures, losing fluorescence
Solution Approach 1:
The patent changes the temperature parameter of the processing method from high-temperature melt-kneading to low-temperature mixing. This parameter change prevents thermal decomposition of temperature-sensitive fluorescent dyes while still achieving uniform dispersion through extended mixing time and mechanical action at lower temperatures.
Solution Approach 2:
The patent performs preliminary mixing of the fluorescent dye with the resin at low temperature before any heating or molding operations. This preliminary action ensures uniform dispersion is achieved before the dye is exposed to conditions that could cause decomposition, preserving fluorescence while achieving uniformity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method provides a more reliable and stable measurement of near-infrared fluorescence intensity by using standardized plates, eliminating variations and ensuring consistent results over time.
Implementation Method 1
near-infrared fluorescent dye that emits near-infrared fluorescence with a maximum fluorescence wavelength within a range from 650 nm to 1000 nm
Data Source
AI summary
The present invention provides a method for measuring a relative fluorescence intensity of a test sample, where a plate-shaped or film-shaped molded body of a resin composition obtained by melt-kneading a raw material mixture containing a near-infrared fluorescent dye and an amorphous resin is used as a standard plate, a plate-shaped or film-shaped molded body of a resin composition having a composition identical to that of the standard plate except that the near-infrared fluorescent dye is not contained is used as a blank plate, the test sample is a molded body of a resin composition containing a near-infrared fluorescent dye, and a molded body having a composition identical to that of the test sample except that the near-infrared fluorescent dye contained in the test sample is not contained is used as a test sample blank, and (F1-F1B)/(FS-FSB) is defined as the relative fluorescence intensity of the test sample (where F1, F1B, FS, and FSB are fluorescence intensities of the test sample, the test sample blank, the standard plate, and the blank plate, respectively, at the near-infrared region).