Nitrogenous Compound Reagent for Biomolecule Lysis and Stabilization
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Solution Overview
Problem
Current methods for analyzing biomolecules, such as nucleic acids and proteins, face challenges in complete lysis of biological samples, stabilization of biomolecules, reduction of inhibiting effects, and differential separation of similar biomolecules like RNA and DNA, which often require additional reconditioning steps and can lead to contamination and cost inefficiencies.
Innovation Solution
A method involving the use of nitrogenous compounds like polyamines, amino acids, and heterocyclic compounds to enhance lysis efficiency, stabilize biomolecules, and reduce inhibiting effects, allowing for improved sample preparation and analysis by maintaining biomolecules in a dissolved or suspended state without precipitation, thereby simplifying subsequent detection methods.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional lysis methods are used to release biomolecules from cells, then biomolecules become accessible for analysis, but additional reconditioning steps are required and contamination risks increase
Solution Approach 1:
The patent combines multiple functions (lysis, stabilization, and inhibition reduction) into a single reagent composition containing nitrogenous compounds. This eliminates the need for separate reconditioning steps that were previously required after conventional lysis methods, thereby reducing processing complexity while maintaining biomolecule accessibility.
Solution Approach 2:
The nitrogenous compound composition serves multiple purposes simultaneously: it acts as a lysis agent to release biomolecules, a stabilizer to preserve biomolecule integrity, and an inhibitor of interfering substances. This multi-functionality reduces the number of separate processing steps needed in conventional methods.
2Reliability
If conventional lysis methods are used to release biomolecules from cells, then biomolecules become accessible for analysis, but contamination risks increase
Solution Approach 1:
The patent converts potentially harmful substances present in biological samples into beneficial components by using nitrogenous compounds that stabilize biomolecules and inhibit interfering substances simultaneously. This approach transforms the complex cellular environment from a source of contamination into a controlled reaction medium.
3Stability of the object's composition
If additional reconditioning steps are performed to stabilize biomolecules, then biomolecule integrity is maintained, but processing time and cost increase
Solution Approach 1:
The nitrogenous compound composition performs stabilization action preliminarily during the lysis process itself, rather than requiring separate post-lysis reconditioning steps. This preliminary stabilization maintains biomolecule integrity while eliminating subsequent processing time.
4Manufacturing precision
If differential separation methods are used to separate RNA and DNA, then specific biomolecules are isolated, but additional processing steps and cost inefficiencies occur
Solution Approach 1:
The patent extracts only the necessary function (biomolecule stabilization and inhibition reduction) from complex differential separation methods. By using nitrogenous compounds that maintain biomolecules in a dissolved or suspended state without precipitation, the method eliminates the need for additional separation steps while preserving the ability to analyze specific biomolecules.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables more effective lysis and stabilization of biomolecules, reduces inhibiting interactions, and simplifies sample preparation, leading to improved detection accuracy and reduced contamination risks, while potentially eliminating the need for additional reconditioning steps.
Implementation Method 1
A method involving the use of nitrogenous compounds like polyamines, amino acids, and heterocyclic compounds to enhance lysis efficiency, stabilize biomolecules, and reduce inhibiting effects, allowing for improved sample preparation and analysis by maintaining biomolecules in a dissolved or suspended state without precipitation
Data Source
AI summary
The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo-oder heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carbonxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more of these compounds. The invention provides in particular a method for the lysis of a biological sample, and methods for the stabilization of biomolecules, a method for the decrease of inhibiting effects in a sample containing biomolecules and a differential masking method.