Nitrosococcus Bacteria Formulation for High Salt Ammonia Treatment
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Solution Overview
Problem
Conventional biological deodorization methods using nitrifying bacteria are insufficient for treating ammonia under high ion concentration conditions, particularly in environments like livestock farms and composting plants, where the activity of bacteria such as Nitrosomonas is significantly reduced.
Innovation Solution
A nitrifying bacteria formulation containing ammonia-oxidizing bacteria from the genus Nitrosococcus and nitrite-oxidizing bacteria, with specific proportions of each, is developed to maintain nitrifying activity and recover nitrate nitrogen from ammonia, even under high ion concentrations, through a method involving culturing these bacteria in a medium with controlled salt and magnesium concentrations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional nitrifying bacteria (e.g., Nitrosomonas) are used for biological deodorization, then ammonia treatment is effective under normal conditions, but nitrifying activity is significantly reduced under high ion concentration conditions
Solution Approach 1:
The invention changes the key parameter of bacterial species composition by selecting halophilic ammonia-oxidizing bacteria (Nitrosococcus halophilus) that are specifically adapted to high salt concentrations, replacing conventional non-halophilic bacteria. This parameter change enables the system to maintain nitrifying activity under high ion concentration conditions where conventional bacteria fail.
2Productivity
If nitrifying bacteria are used to treat ammonia, then nitrate nitrogen can be recovered, but treatment efficiency is insufficient under high salt concentration conditions
Solution Approach 1:
The invention changes the environmental parameter (salt concentration) that the bacteria are adapted to handle, by selecting halophilic species that not only tolerate but thrive in high salt conditions. This enables productive ammonia treatment in environments previously unsuitable for nitrification.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The formulation enables effective nitrification reactions and efficient recovery of high-concentration nitrate nitrogen in environments with high ion concentrations, such as sea water, improving ammonia treatment efficiency and reducing odor complaints.
Implementation Method 1
nitrifying bacteria decompose ammonia by oxidizing it to nitrous acid and further to nitric acid
Implementation Method 2
nitrifying bacteria decompose ammonia by oxidizing it to nitrous acid and further to nitric acid
Data Source
AI summary
A nitrifying bacteria formulation that can cause a sufficient nitrification reaction even in a high ionic concentration (salt concentration), and efficiently collect high-concentration nitrate-form nitrogen from ammonia produced in animal husbandry facilities, composting facilities, sewage treatment plants, etc.; and a method for culturing bacteria to be contained in the nitrifying bacteria formulation. A nitrifying bacteria formulation includes: ammonia-oxidizing bacteria and nitrite-oxidizing bacteria, wherein the ammonia-oxidizing bacteria include bacteria belonging to the Nitrosococcus, the proportion of the number of the bacteria belonging to the Nitrosococcus with respect to the total number of bacteria in the nitrifying bacteria formulation is at least 0.1%, and the proportion of the number of the nitrite-oxidizing bacteria with respect to the total number of bacteria in the nitrifying bacteria formulation is at least 0.1%. A method for culturing bacteria to be contained in the nitrifying bacteria formulation may also be provided.