NKT-like Cell Expansion Using Allogeneic Somatic Cells
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Solution Overview
Problem
Current methods for activating and expanding CD8+ NKT-like cells in vitro are inefficient and require the addition of cytokines, limiting large-scale expansion and activation of these cells, which are crucial for their antitumor effects.
Innovation Solution
A method involving co-culture of lymphocytes from a first subject with nucleated somatic cells from a second allogeneic or heterogeneous subject, expressing immune co-stimulatory molecules, to expand CD8+ NKT-like cells by 10 to 1000 times, without the need for cytokine addition, and enhance their activation markers and killing effector molecules.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If cytokines are added to expand NKT-like cells, then cell activation and expansion can be achieved, but the process becomes complex and limits large-scale expansion
Solution Approach 1:
The patent extracts and removes the requirement for cytokine addition from the cell expansion process. By using allogeneic nucleated somatic cells as a natural stimulator, the method eliminates the need for external cytokine supplementation, thereby simplifying the culture process while maintaining effective NKT-like cell expansion and activation
Solution Approach 2:
The allogeneic nucleated somatic cells naturally provide the necessary co-stimulatory signals and cytokines through their inherent biological functions. The system becomes self-sufficient, where the somatic cells themselves generate the required biological environment for NKT-like cell expansion without external intervention, reducing process complexity
2Quantity of substance
If current activation methods are used, then some cell expansion is achieved, but large-scale expansion is limited
Solution Approach 1:
The patent performs preliminary action by pre-preparing allogeneic nucleated somatic cells before the actual NKT-like cell expansion. These somatic cells are cultured and characterized in advance to ensure they possess the necessary co-stimulatory properties, enabling them to effectively drive large-scale NKT-like cell expansion when introduced to the lymphocyte culture
Solution Approach 2:
The allogeneic nucleated somatic cells serve as an intermediary that mediates the expansion of NKT-like cells. Rather than directly stimulating NKT-like cells with complex cytokine mixtures, the somatic cells act as a natural bridge, providing physiological co-stimulatory signals that result in more efficient and scalable cell expansion
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method simplifies and enhances the expansion and activation of CD8+ NKT-like cells, increasing their antitumor effects by significantly increasing cell numbers and expression of activation markers like CD25, CD44, CD69, Granzyme B, and Perforin.
Implementation Method 1
nucleated somatic cells from a second allogeneic or heterogeneous subject, expressing immune co-stimulatory molecules
Data Source
AI summary
The invention relates to a method for activating and expanding NKT-like cells in vitro. The method according to the present invention utilizes nucleated somatic cells derived from allogeneic or heterologous subjects to activate and proliferate NKT-like cells of a subject in vitro, and can increase the overall number of the NKT-like cells, the expression of activation markers and the number of killing effector molecules.