NMR Spectroscopy Detection of Abnormal Lipoproteins in Blood Plasma
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Solution Overview
Problem
Current methods for detecting Lipoprotein X (LP-X) and other abnormal lipoproteins in blood plasma or serum are laborious, difficult to quantify, and often misdiagnose conditions, leading to inappropriate treatments for patients with liver disease or LCAT deficiency.
Innovation Solution
The use of Nuclear Magnetic Resonance (NMR) spectroscopy to accurately detect and quantify LP-X and other abnormal lipoproteins by applying deconvolution models that account for unique spectral signatures of LP-X, LP-Y, and LP-Z, allowing for precise identification and quantification in biosamples.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If electrophoresis procedures are used to identify LP-X, then detection capability is achieved, but the process becomes laborious and quantification becomes difficult
Solution Approach 1:
The patent replaces the mechanical electrophoresis system with an NMR spectroscopy system. NMR uses magnetic fields and radiofrequency pulses to detect lipoprotein characteristics, eliminating the need for manual electrophoresis operations while providing both qualitative identification and quantitative measurement of LP-X and other abnormal lipoproteins
Solution Approach 2:
The patent changes the detection parameter from electrophoretic mobility to NMR spectral characteristics. By analyzing specific spectral regions and patterns in NMR spectra, the system can identify and quantify abnormal lipoproteins automatically, improving both detection accuracy and testing efficiency
2Ease of operation
If standard cholesterol screening techniques are used, then routine testing is simple, but LP-X detection capability is lost leading to misdiagnosis
Solution Approach 1:
The patent segments the NMR spectrum into multiple regions, with specific attention to spectral regions characteristic of abnormal lipoproteins. By analyzing these segmented regions alongside standard cholesterol parameters, the system maintains routine testing simplicity while adding diagnostic capability for LP-X detection
Solution Approach 2:
The NMR system performs multiple functions simultaneously: it measures standard lipoprotein cholesterol levels and particle sizes while also detecting abnormal lipoproteins like LP-X. This multi-functionality is achieved through comprehensive spectral analysis that identifies both normal and abnormal lipoprotein characteristics in a single test
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
NMR spectroscopy enables rapid, accurate detection and quantification of LP-X and other abnormal lipoproteins, improving diagnostic accuracy and preventing inappropriate treatments by providing a reliable method for routine cholesterol screening and monitoring treatment responses.
Implementation Method 1
NMR spectroscopy enables rapid, accurate detection and quantification of LP-X and other abnormal lipoproteins
Data Source
AI summary
Described herein are methods and systems for the determination of constituents in biosamples by NMR spectroscopy and more specifically for the determination of lipoprotein constituents LP-X, LP-Y, and LP-Z in blood plasma and serum.