NMR Spectroscopy Biomarker Detection for SARS-CoV-2 Diagnosis

The patent applies parameter changes by utilizing multiple NMR spectroscopy parameters including diffusion coefficients, relaxation times (T1 and T2), and chemical shift values to characterize plasma metabolites. By measuring these physical and chemical parameters of molecular interactions, the method achieves high diagnostic accuracy for SARS-CoV-2 infection and inflammatory conditions without requiring complex additional equipment, thus resolving the contradiction between measurement precision and device complexity.

The patent replaces mechanical separation methods with non-invasive NMR spectroscopy that interrogates molecular interactions in the native plasma state. The method uses pulse sequences (such as diffusion-edited, relaxation-edited, and combined DIRE) to extract diagnostic information about metabolites and molecular dynamics without physically altering or consuming the sample, thereby maintaining measurement precision while preserving the sample for further analysis.

The patent merges multiple NMR pulse sequences (diffusion-edited, relaxation-edited, and combined DIRE) into an integrated measurement approach that simultaneously extracts multiple types of biomarker information including metabolite concentrations, molecular mobility, and relaxation characteristics. By combining these methods rather than applying them sequentially, the patent enriches diagnostic information while minimizing total measurement time, thus resolving the contradiction between information extraction and time loss.