Non-Fluorescent Quencher Composition for Broad-Spectrum Fluorescence Quenching
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Solution Overview
Problem
Existing fluorescent quenchers are limited by narrow wavelength ranges, restricting their compatibility with various fluorescent compounds due to mechanisms like FRET requiring spectral overlap, and ground state quenching not addressing wide wavelength ranges.
Innovation Solution
A non-fluorescent compound capable of both static and dynamic quenching, represented by Chemical Formula 1, which can form ground state complexes and undergo FRET, allowing quenching across a broad wavelength range.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a single quencher is designed to induce FRET-mediated quenching, then quenching efficiency within a specific wavelength range is improved, but the effective wavelength range is narrowed
Solution Approach 1:
The patent applies universality by designing a single quencher molecule that can perform multiple quenching functions across different wavelength ranges. The BODIPY-based quencher structure is engineered to simultaneously enable FRET-mediated quenching for fluorescent compounds with emission spectra overlapping its absorption band and ground state quenching for fluorescent compounds with emission spectra outside its absorption band, making it universally applicable across a broad wavelength range from 500-700 nm
Solution Approach 2:
The patent employs composite materials by combining multiple quenching mechanisms within a single molecular framework. The BODIPY core structure is复合 with specific substituents that enable both FRET and ground state quenching pathways to operate simultaneously, creating a composite quenching system that overcomes the limitations of single-mechanism quenchers
2Adaptability or versatility
If ground state quenching is used without spectral overlap requirements, then compatibility with various fluorescent compounds is improved, but the wavelength range coverage remains limited
Solution Approach 1:
The patent merges two distinct quenching mechanisms—FRET-mediated quenching and ground state quenching—into a single operational system. The BODIPY quencher is designed so that its absorption spectrum enables FRET with fluorescent compounds having emission spectra in the 500-700 nm range, while simultaneously maintaining ground state quenching capability for compounds with emission spectra outside this range, thereby merging the advantages of both mechanisms
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables quenching across a wide wavelength range (200-1000 nm) with various fluorescent compounds, enhancing compatibility and versatility.
Implementation Method 1
representative examples of dynamic quenching include fluorescence resonance energy transfer (FRET)
Implementation Method 2
A representative example of static quenching is ground state quenching
Data Source
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AI summary
The present invention relates to a non-fluorescent compound that is useful as a quencher for a fluorescent compound exhibiting emission properties at an excited energy level, and to various applications thereof.