Nonplanar Solid Substrate Microorganism Separation
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Solution Overview
Problem
Current methods for separating microorganisms from samples rely on specific ligands or external driving forces, and there is a lack of methods utilizing the properties of solid supports and liquid medium conditions, as well as removal of materials preventing cell binding.
Innovation Solution
A method involving contact between a sample containing microorganisms and an inorganic ion exchange material to react and remove inhibitory substances, followed by capture using a non-planar solid support with a larger surface area and specific pH conditions to enhance microorganism attachment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If specific ligands or external driving forces are used for cell separation, then separation selectivity is improved, but device complexity and cost increase
Solution Approach 1:
The invention extracts and removes inhibitory substances from the sample using ion exchange materials, eliminating the need for complex ligand-based separation systems. By removing the harmful factors (inhibitory substances) that prevent cell binding, the system achieves separation using only the inherent properties of the solid support surface, thereby reducing device complexity while maintaining separation capability
Solution Approach 2:
The solid support surface performs separation automatically through its inherent properties after inhibitory substances are removed. The system uses the natural binding characteristics of the solid support for microorganisms without requiring external driving forces or complex ligand systems, allowing the system to serve itself and achieve separation through simplified means
2Productivity
If ion exchange materials are used to remove inhibitory substances, then microorganism capture efficiency is improved, but additional processing steps are required
Solution Approach 1:
The invention merges the sample container with the ion exchange material by placing the material directly in the container. This integration allows the ion exchange pretreatment and subsequent microorganism capture to occur in a unified system, reducing process complexity while maintaining high capture efficiency through the removal of inhibitory substances
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach effectively separates microorganisms by removing inhibitory substances and optimizing binding conditions, leading to improved capture efficiency and purity of microorganisms for subsequent processes like DNA isolation.
Implementation Method 1
contacting a sample containing microorganisms with an inorganic on exchange material such that the sample reacts with the inorganic on exchange material
Implementation Method 2
contacting the reacted sample with a means for capturing microorganisms such that microorganisms in the sample are captured from the sample
Data Source
AI summary
Provided is a method of separating microorganisms from a sample including contacting the sample containing microorganisms with an inorganic on exchange material such that the sample reacts with the inorganic on exchange material, and contacting the reacted sample with a means for capturing microorganisms.