Nonwoven Filter for Rapid Microorganism Detection
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Solution Overview
Problem
Current methods for detecting microorganisms in clinical samples are often slow, costly, and limited by sensitivity, particularly in clinical settings, and face challenges with interference from treatment chemicals and the need for large sample volumes.
Innovation Solution
A device and method utilizing a nonwoven article with a filter holder and adaptor to concentrate microorganisms from fluid samples, allowing for rapid detection using a detection reagent and luminometer, enabling the detection of microorganisms in small volumes within 15 minutes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Speed
If ATP bioluminescence assays are used for rapid detection, then detection speed is improved, but detection sensitivity deteriorates due to requiring at least 1×10^5 cfu/ml
Solution Approach 1:
The nonwoven article performs preliminary concentration of microorganisms from the fluid sample before detection. By filtering and concentrating the sample through the nonwoven matrix, the method prepares the sample in advance to achieve sufficient concentration for sensitive detection, resolving the contradiction between rapid detection and detection sensitivity
Solution Approach 2:
The nonwoven article acts as an intermediary between the fluid sample and the detection reagent. It concentrates microorganisms from large volumes of fluid and presents them in a concentrated form to the detection system, enabling both rapid and sensitive detection without requiring large sample volumes
2Measurement precision
If large sample volumes are used to increase detection sensitivity, then detection sensitivity is improved, but device complexity and ease of operation worsen
Solution Approach 1:
The nonwoven article provides localized concentration capability within a compact device structure. Rather than requiring complex large-volume handling systems, the concentration function is localized to the nonwoven matrix where microorganisms are trapped and concentrated from passing fluid, maintaining simplicity while achieving high sensitivity
Solution Approach 2:
The nonwoven article utilizes its porous structure to concentrate microorganisms from fluid samples. The porous matrix allows fluid to pass through while trapping and concentrating microorganisms, enabling sensitive detection in a simple, compact device without complex mechanisms
3Measurement precision
If antibody-coated particles are used for specific bacterial concentration, then detection sensitivity is improved, but cost increases
Solution Approach 1:
The nonwoven article serves as a disposable, cost-effective alternative to expensive antibody-coated particles. While it may provide non-specific or less specific concentration compared to antibody-based methods, it achieves sufficient sensitivity for clinical applications at a much lower cost, making the testing process economically viable
Solution Approach 2:
The method changes the concentration parameter by using physical filtration through nonwoven materials rather than specific biological binding. This parameter change allows concentration of microorganisms based on physical properties (size, shape) rather than specific antigen-antibody interactions, reducing cost while maintaining adequate sensitivity for detection
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enables rapid and sensitive detection of microorganisms in fluid samples, reducing the need for large sample volumes and minimizing interference, making it suitable for clinical applications.
Implementation Method 1
passing a fluid sample suspected of containing at least one microorganism strain or target cellular analyte from the lumened or cannulated device through the device and contacting the nonwoven article
Implementation Method 2
contacting the nonwoven article with at least one detection reagent and detecting the presence of the at least one microorganism strain or target cellular analyte concentrated by the nonwoven article
Implementation Method 3
Rapid detection methods based on ATP bioluminescence assays have been used to determine microbial contamination in fluids such as water
Data Source
AI summary
Devices for detecting microorganism strains or target cellular analytes are provided. The device includes a filter holder, the filter holder comprising a tip portion; a nonwoven article disposed on the tip portion of the filter holder; and an adaptor attacked to the filter holder, the adaptor defining an aperture. Methods of detecting microorganisms and/or cellular analytes in a fluid sample using the devices are also provided. The method includes obtaining the device; placing a lumened or cannulated device in fluid communication with the device; and passing a fluid sample suspected of containing at least one microorganism strain or target cellular analyte from the lumened or cannulated device through the device and contacting the nonwoven article. The method further includes contacting the nonwoven article with at least one detection reagent and detecting the presence of the at least one microorganism strain or target cellular analyte concentrated by the nonwoven article. Kits and systems including the devices are also provided.