Novel Polypeptide for Industrial Polyester Degradation

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Solution Overview

Problem

Current methods for degrading polyester-containing plastics are inefficient and costly, particularly for single-use disposable applications, and existing enzymatic methods lack effective industrial-scale solutions for degrading polyesters in film or pellet form.

Innovation Solution

A novel polypeptide derived from Actinomadura sp. with enhanced thermostability and polyester-degrading activity is identified, allowing for the industrial-scale degradation of polyesters, including polylactic acid, and the production of reusable monomers and oligomers.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If mechanical recycling processes are used to reprocess plastic material, then recycling can be achieved, but huge amounts of electricity are consumed and equipment costs are expensive

Engineering Contradiction:
Improverecycling capabilityVSAvoidelectricity consumption
Core Design Contradiction:
ProductivityVSUse of energy by moving object

Solution Approach 1:

The patent replaces mechanical recycling processes with enzymatic degradation. Instead of using mechanical extrusion equipment that consumes huge amounts of electricity, the invention uses isolated enzymes (proteases) to chemically degrade polyester plastics into monomers, thereby substituting mechanical energy with biochemical catalysis to achieve recycling

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Productivity

If chemical recycling processes are used to recover chemical constituents, then monomers can be recovered, but the process is expensive and not efficient on raw plastic products

Engineering Contradiction:
Improvemonomer recoveryVSAvoidprocess cost
Core Design Contradiction:
ProductivityVSEase of manufacture

Solution Approach 1:

The patent replaces expensive chemical recycling processes with enzymatic degradation. Instead of complex chemical processes that require purified polymers and expensive reagents, the invention uses isolated enzymes to efficiently degrade raw polyester plastics (films, pellets, mixed plastics) into monomers under milder conditions, reducing both cost and complexity

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent changes the parameters of the recycling process by using enzymatic catalysis instead of harsh chemical conditions. The enzymes operate at moderate temperatures and pH levels, transforming the process from high-cost chemical recycling to cost-effective biological recycling that works on raw plastic products

Inventive Principle:
Principle #35Parameter changes

3Productivity

If existing enzymatic degradation methods are used, then plastic degradation can occur, but the enzymes only work on emulsion form and have poor degrading ability

Engineering Contradiction:
Improvedegradation capabilityVSAvoidsubstrate form compatibility
Core Design Contradiction:
ProductivityVSAdaptability or versatility

Solution Approach 1:

The patent replaces existing enzymatic methods with improved isolated enzymes. Instead of using crude enzyme extracts that only work on emulsions, the invention uses purified and isolated protease enzymes that maintain high activity on various substrate forms including films, pellets, and mixed plastics, thereby improving both degradation capability and substrate adaptability

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent extracts and isolates the active degradative enzymes from their natural sources. By isolating the specific protease enzymes responsible for polyester degradation and purifying them, the invention creates enzymes with enhanced stability and broader substrate compatibility, removing the limitations of crude enzyme preparations

Inventive Principle:
Principle #2Taking out (Extraction)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The novel polypeptide effectively degrades polyester plastics, enabling the recycling of polyesters on an industrial scale, reducing environmental impact and providing a cost-effective alternative to existing recycling methods.

Implementation Method 1

enzymes can accelerate hydrolysis of plastics

Methodology Applied
Scientific EffectHydrolysis: Hydrolysis

Implementation Method 2

The polypeptide of the invention is particularly suited to degrade polylactic acid, and material containing polylactic acid, as plastic material

Methodology Applied
Scientific EffectEnzyme catalysis: Enzyme

Data Source

PatentEP3209771B1Polypeptide having a polyester degrading activity and uses thereof
Publication Date: 2020.10.14 INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE
  • EP3209771B1 patent drawingFigure 1~2
  • EP3209771B1 patent drawingFigure 3~4
  • EP3209771B1 patent drawingFigure 5~6

AI summary

The present invention relates to a new isolated polypeptide comprising an amino acid sequence having at least 94%, 95%, 99% or 100% identity to the full length amino acid sequence set forth in SEQ ID N°1, and having a polyester degrading activity, and uses thereof.