Novel Quencher with Multiple Reactive Groups for Biomolecule Binding
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Solution Overview
Problem
Conventional quenchers used in biology for fluorescence control have limited reactive groups for binding to biomolecules, leading to stability issues and restricted applications, especially in water-soluble conditions, with limited options for reactive groups that maintain long-term reaction and storage stability.
Innovation Solution
A novel quencher compound with specific moieties and reactive groups, such as carboxyl, hydroxyl, and sulfonyl halides, is developed to enhance quenching efficiency and stability, allowing for effective binding to biomolecules and improved performance in optical imaging and nucleic acid detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Device complexity
If conventional quenchers with limited reactive groups are used, then the structure is simple, but the stability and versatility in water-soluble conditions deteriorate
Solution Approach 1:
The quencher employs a composite molecular structure combining multiple reactive groups (carboxyl, hydroxyl, sulfonyl halide) within a single molecule, creating a multifunctional compound that simultaneously provides water solubility, stability, and versatile binding capability to different biomolecules
Solution Approach 2:
The quencher is designed with multiple types of reactive groups that can bind to different functional groups on various biomolecules (amines, thiols, hydroxyls, carboxyls), making it universally applicable across different biological systems and conditions while maintaining stability in water-soluble environments
2Ease of manufacture
If conventional quenchers with limited reactive groups are used, then the synthesis is simple, but the selectivity and reaction rate deteriorate
Solution Approach 1:
The quencher molecule is segmented into distinct functional regions with specific reactive groups (carboxyl at one end, hydroxyl in the middle, sulfonyl halide at the other end), allowing selective engagement with different biomolecule targets while maintaining overall molecular stability and synthetic feasibility
Solution Approach 2:
Different regions of the quencher molecule possess different chemical properties and reactivities - the carboxyl group provides acidic properties, the hydroxyl group provides hydrogen bonding capability, and the sulfonyl halide provides high reactivity toward nucleophiles, enabling localized selective binding to specific biomolecule targets
3Quantity of substance
If conventional quenchers are used, then the cost is low, but the quenching efficiency and detection precision deteriorate
Solution Approach 1:
The quencher optimizes key molecular parameters including the introduction of heavy atoms (iodine, bromine) to enhance spin-orbit coupling and promote intersystem crossing, increasing the triplet state population and thereby improving quenching efficiency and detection sensitivity without sacrificing availability
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The novel quencher exhibits high quenching efficiency and stability, enabling effective fluorescence control and improved detection of biomolecules, particularly nucleic acids, with enhanced reactive group selectivity and stability compared to conventional quenchers.
Implementation Method 1
A quenching phenomenon is known to occur through a mechanism including fluorescence resonance energy transfer (FRET), photo-induced electron transfer and dye coagulation such as H-dimer formation.
Implementation Method 2
A quenching phenomenon is known to occur through a mechanism including fluorescence resonance energy transfer (FRET), photo-induced electron transfer and dye coagulation such as H-dimer formation.
Data Source
AI summary
The present invention relates to a quencher which exhibits a quenching effect on a fluorescent material exhibiting luminescence at an excited energy level, and various uses thereof.