NPAA2720 Maize Inbred Line for Regional Adaptation

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Solution Overview

Problem

Corn breeding faces challenges in producing high-yielding, agronomically sound plants that are uniformly adapted across the U.S. Corn Belt, as regional growing conditions and specific issues like Gray Leaf Spot infection, cool temperatures, and soil pH levels require tailored traits in hybrids, which existing methods struggle to address effectively.

Innovation Solution

The development of the maize inbred plant NPAA2720, which includes a mutant or transgenic gene conferring traits such as herbicide resistance, insect resistance, disease resistance, and abiotic stress tolerance, allowing for the production of hybrid seeds through crossing with other maize plants and subsequent backcrossing to introduce desired traits, ensuring adaptability and performance across various Corn Belt regions.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional breeding methods are used to develop maize hybrids, then uniformity and reproducibility are achieved, but adaptability to diverse regional conditions and resistance to specific diseases like Gray Leaf Spot are insufficient

Engineering Contradiction:
Improveadaptability to diverse regional conditionsVSAvoidresistance to specific diseases
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The patent applies local quality by developing inbred lines with specific regional adaptations. Different inbred lines are bred to possess traits optimized for particular Corn Belt regions, including resistance to region-specific diseases like Gray Leaf Spot in the eastern Corn Belt, tolerance to cool temperatures in northern regions, and adaptation to high pH soils in western regions. This allows hybrids to be tailored for local conditions while maintaining the uniformity of inbred-based breeding.

Inventive Principle:
Principle #3Local quality

Solution Approach 2:

The patent uses composite materials by creating hybrid combinations that integrate multiple inbred lines with complementary traits. The hybrid NPAA2720 and its counterparts combine desirable characteristics from different parental inbreds, including disease resistance, stress tolerance, and yield potential, resulting in a composite genetic structure that addresses multiple regional challenges simultaneously.

Inventive Principle:
Principle #40Composite materials

2Manufacturing precision

If inbred lines are used as parents for hybrid production, then uniformity and reproducibility of hybrid seed are achieved, but the inbred plants themselves show decreased vigor and are difficult to produce

Engineering Contradiction:
Improveuniformity of hybrid seed productionVSAvoidvigor of inbred plants
Core Design Contradiction:
Manufacturing precisionVSProductivity

Solution Approach 1:

The patent applies blessing in disguise by accepting the reduced vigor of inbred plants as an unavoidable consequence of homozygosity, but converting this limitation into a benefit through hybridization. The inbred lines, while less vigorous individually, serve as uniform parental stocks that produce highly vigorous and uniform hybrid offspring. The homozygosity that reduces inbred vigor ensures genetic uniformity in the parents, which in turn ensures reproducibility of the hybrid seed production process.

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

3Productivity

If hybrids are developed to show increased vigor and yield, then productivity is improved, but the grain produced by hybrid plants (F2 grain) comprises segregating germplasm and loses uniformity

Engineering Contradiction:
Improveyield and vigor of hybrid plantsVSAvoiduniformity of F2 grain
Core Design Contradiction:
ProductivityVSManufacturing precision

Solution Approach 1:

The patent applies preliminary action by performing emasculation of the female parent before pollination to prevent self-pollination and maintain hybrid uniformity. The female inbred line is emasculated (removal of tassels or chemical treatment) before the hybrid seed production process, ensuring that only controlled cross-pollination from the male parent occurs. This preliminary action prevents the segregation that would otherwise occur in F2 grain and ensures that the harvested seed remains true-to-type F1 hybrid seed.

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS8710335B2Variety corn line NPAA2720
Publication Date: 2014.04.29 SYNGENTA CROP PROTECITON AG

AI summary

The present invention provides an inbred corn line designated NPAA2720, methods for producing a corn plant by crossing plants of the inbred line NPAA2720 with plants of another corn plant. The invention further encompasses all parts of inbred corn line NPAA2720, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line NPAA2720, and plants produced according to these methods.