Inbred Corn Line NPID2001 Marker-Assisted Breeding

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Solution Overview

Problem

Corn breeding faces challenges in producing high-yielding, agronomically sound plants that are uniformly adapted across different regions of the Corn Belt, as existing methods struggle to combine desirable traits like yield, disease resistance, and adaptability without significant genetic complexity and the need for extensive breeding.

Innovation Solution

The development of the inbred corn line NPID2001, which includes specific traits and transgenic genes for improved starch, disease, and insect resistance, along with a method for producing hybrid seeds using this line and another inbred line, allowing for targeted trait introduction through marker-assisted breeding to enhance hybrid performance across various environments.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional corn breeding methods are used to develop inbreds with desirable traits, then trait combination is achieved, but breeding complexity and time are significantly increased

Engineering Contradiction:
Improvetrait combinationVSAvoidbreeding complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent replaces traditional mechanical breeding methods (manual crossing, selection, and phenotypic evaluation) with molecular marker-based identification systems. Specific DNA markers are associated with desirable traits, allowing breeders to identify and select plants with desired traits through genetic analysis rather than extensive phenotypic observation and manual crossing, thereby reducing breeding complexity while achieving the same trait combinations.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Adaptability or versatility

If extensive breeding processes are used to combine desirable traits, then trait combination is achieved, but time consumption increases significantly

Engineering Contradiction:
Improvetrait combinationVSAvoidbreeding time
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent employs preliminary action by pre-identifying and characterizing molecular markers that are linked to desirable traits before the actual breeding process. These markers serve as predictive indicators, allowing breeders to select plants with desired traits at an early stage without waiting for phenotypic expression or conducting multiple generations of breeding trials, thereby significantly reducing the time required to achieve desired trait combinations.

Inventive Principle:
Principle #10Preliminary action

3Stability of the object's composition

If inbred lines are developed through self-pollination, then homozygosity is achieved, but plant vigor decreases

Engineering Contradiction:
ImprovehomozygosityVSAvoidplant vigor
Core Design Contradiction:
Stability of the object's compositionVSReliability

Solution Approach 1:

The patent replaces traditional phenotypic selection methods with molecular marker-based selection during the inbreeding process. By using DNA markers to identify and select for homozygosity at specific loci associated with vigor and other desirable traits, breeders can maintain plant vigor while achieving the necessary homozygosity for stable inbred line development, overcoming the traditional trade-off between homozygosity and vigor.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Data Source

PatentUS8389821B1Inbred corn line NPID2001
Publication Date: 2013.03.05 SYNGENTA CROP PROTECITON AG

AI summary

Basically, this invention provides for an inbred corn line designated NPID2001, methods for producing a corn plant by crossing plants of the inbred line NPID2001, with plants of another corn plant. The invention relates to the various parts of inbred NPID2001, including culturable cells. This invention also relates to methods for introducing transgenic transgenes into inbred corn line NPID2001, and plants produced by said methods.